| 一株金龟子绿僵菌对茶尺蠖的致病力和胞外酶活性 |
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| 引用本文： | 陈峰,王长方,王俊,陈文乐,胡进锋,吴玮,姚锦爱. 一株金龟子绿僵菌对茶尺蠖的致病力和胞外酶活性[J]. 中国生物防治学报, 2021, 37(1): 124-129. DOI: 10.16409/j.cnki.2095-039x.2021.01.023 |
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| 作者姓名： | 陈峰 王长方 王俊 陈文乐 胡进锋 吴玮 姚锦爱 |
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| 作者单位： | 1. 福建省作物有害生物监测与治理重点实验室/福建省农业科学院植物保护研究所, 福州 350013;2. 三明市农业农村局, 三明 365000 |
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| 基金项目： | 福建省科技计划公益类专项(2018R1025-6,2017R1025-8);福建省农业科学院科技服务团队项目(kjfw04)。 |
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| 摘 要： | 为明确从茶园腐殖层昆虫尸体上分离的虫生真菌的分类地位及其对茶尺蠖的生防潜力,采用形态学特征观察和rDNA?ITS序列分析方法对该菌株进行鉴定,并测定该菌株对茶尺蠖4龄幼虫的毒力及其胞外酶活性.结果表明,从茶园腐殖层虫尸上分离获得一株金龟子绿僵菌CHMA?005;该菌株侵染茶尺蠖4龄幼虫10 d后,致死率、LC50和LT...
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| 关 键 词： | 金龟子绿僵菌 毒力 胞外酶活性 茶尺蠖 |
| 收稿时间： | 2020-05-07 |
Pathogenicity and Extracellular Enzyme Activities of Metarhizium anisopliae to Ectropis obliqua hypulina Wehrli |
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| CHEN Feng,WANG Changfang,WANG Jun,CHEN Wenle,HU Jinfeng,WU Wei,YAO Jinai. Pathogenicity and Extracellular Enzyme Activities of Metarhizium anisopliae to Ectropis obliqua hypulina Wehrli[J]. Chinese Journal of Biological Control, 2021, 37(1): 124-129. DOI: 10.16409/j.cnki.2095-039x.2021.01.023 |
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| Authors: | CHEN Feng WANG Changfang WANG Jun CHEN Wenle HU Jinfeng WU Wei YAO Jinai |
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| Affiliation: | 1. Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests/Institute of Plant Protection, Fujian Academy of Agricultural Science, Fuzhou 350013, China;2. Sanming Agricultural and Rural Bureau, Sanming 365000, China |
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| Abstract: | Fungal strains were identified using morphological characteristics and rDNA-ITS sequence from entomogenous fungi isolated from insect cadaver in the humic layer of tea garden and evaluated for their biocontrol potential and extracellular enzyme activities against the 4th instar larvae of Ectropis obliquus.The isolated fungus was identified as Metarhizium anisopliae CHMA-005.After infection of the 4th instar larvae of E.obliquus for 10 days,the strain CHMA-005 resulted in mortality,LC50 and LT50 of 100%,1.84×104 cfu/mL and 3.57 d,respectively.Protease activity and chitinase activity peaked on the 6th day after infection,which were 31.43 U/mL and 27.73 U/mL,respectively.Therefore,with high pathogenicity and extracellular enzyme activities to E.obliqua,M.anisopliae strain CHMA-005 has good biocontrol potential and application prospect. |
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| Keywords: | Metarhizium anisopliae toxicity extracellular enzyme activity Ectropis oblique |
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