大草蛉雄虫卵黄原蛋白基因的鉴定和表达特征

为探索卵黄原蛋白基因对大草蛉雄虫生殖生理等活动的调节作用,利用PCR技术克隆了大草蛉卵黄原蛋白基因部分cDNA序列,采用生物信息学方法分析其序列特征,通过实时荧光定量PCR技术检测了该基因在不同发育阶段和不同组织的表达特征.结果表明,克隆到的卵黄原蛋白基因的cDNA长度为1501 bp,编码501个氨基酸,其预测的蛋白...

Identification and Expression Characteristics of Vitellogenin Gene in Chrysopa pallens Males

In order to explore the regulatory effect of vitellogenin gene on the reproductive physiology and other activities of male Chrysopa pallens,the partial sequence of vitellogenin gene was cloned by PCR,the sequence characteristics were analyzed by bioinformatics,and the expression characteristics of vitellogenin gene in different developmental stages and different tissues were detected by real-time fluorescence quantitative PCR.The results showed that the cDNA length of the cloned vitellogenin gene was 1501 bp,encoding 501 amino acids,the predicted molecular weight of the protein was 206 kD,the isoelectric point was 8.11,and it had vitellogenin conserved cysteine residues,GL/ICG,RXXR and other domains.Sequence analysis showed that the amino acid sequence of C.pallens vitellogenin was not very consistent with that of other insect homologous proteins,which was about 29.46%and 36.43%,which was closely related to the phylogeny of Triatoma infestans.The results of fluorescence quantitative PCR showed that there was no significant difference in the expression of vitellogenin in different ages of adults,but the expression in the abdomen of males was higher than that in the head and chest,and there was no significant difference between the head and chest,but the expression was low.The results showed that the expression of vitellogenin gene in C.pallens was tissue-specific,and it was speculated that it might be involved in the reproductive regulation of male of C.pallens.