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| 呋喃丹降解菌的分离与特性研究 | |
| 引用本文： | 李玉梅,王根林,于洪久,汤晖,陶波,马凤鸣.呋喃丹降解菌的分离与特性研究[J].大豆科学,2009,28(2). |
| 作者姓名： | 李玉梅 王根林 于洪久 汤晖 陶波 马凤鸣 |
| 作者单位： | 1. 东北农业大学农学院,黑龙江,哈尔滨,150010;黑龙江省农业科学院农村能源研究所,黑龙江,哈尔滨,150086 2. 黑龙江省农业科学院土肥所,黑龙江,哈尔滨,150086 3. 黑龙江省农业科学院农村能源研究所,黑龙江,哈尔滨,150086 4. 河北大学生命科技学院,河北,保定,071002 5. 东北农业大学农学院,黑龙江,哈尔滨,150010 |
| 基金项目： | 黑龙江省农科院青年基金 |
| 摘 要： | 采用富集驯化培养方法,从长期施用呋喃丹的大豆田土壤中分离到1株可高效降解呋喃丹的菌株.生物降解表明,5 d内该菌株生物降解农药牢达到94.7%;降解后期培养液采用气质联用分析方法末能检测到原药呋喃丹,检测到有新的化合物生成,而对照培养液中末发现有该类化合物的生成.同时无机盐培养液后期有红色现象生成,可能与呋喃酚开环有关;无细胞提取液加入农药重新培养表明,菌株可通过分泌胞外诱导酶在周质空间内降解农药;经16S rDNA鉴定,初步认为该菌株属于沙雷氏菌属(Serratia sp.). |
| 关 键 词： | 呋喃丹 生物降解 沙雷氏菌属 |
Separating and Characteristics of a Strain Capable of Degrading Carbofuran |
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| LI Yu-mei,WANG Gen-lin,YU Hong-jiu,TANG Hui,TAO Bo,MA Feng-ming.Separating and Characteristics of a Strain Capable of Degrading Carbofuran[J].Soybean Science,2009,28(2). | |
| Authors: | LI Yu-mei WANG Gen-lin YU Hong-jiu TANG Hui TAO Bo MA Feng-ming |
| Institution: | LI Yu-mei1,2,WANG Gen-lin2,YU Hong-jiu2,TANG Hui3,TAO Bo1,MA Feng-ming1(1Agricultural College of North-East Agricultural University,Harbin 150010,Heilongjiang,2Country Resource Institute of Heilongjiang Academy of Agricultural Sciences,Harbin 150086,3Soil Fertilizer Institute of Heilongjiang Academy of Agricultural Sciences,4College of Life Science,Hebei University,Baoding 071002,Hebei,China) |
| Abstract: | One strain that is capable of degrading carbofuran was isolated from the carbofuran acclimated soybean soil by domestication and enrichment.Biodegrading test showed the degraded ration reached 94.7% in five days.By GC-MS analyzing,carbofuran was degraded into carbophenyl mostly,and in inorganic medium,red compound of carol was showed after a given periods,which may be related with the opening loop of carbophenyl.In no cell extracting solution with pesticide,carbofuran was also degraded into carbophenyl,whic... |
| Keywords: | Carbofuran Biodegradation Serratia sp |
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