患“白体病”罗氏沼虾腹部肌肉病变的超微结构

取患“白体病”罗氏沼虾 (Macrobrachiumrosenbergii)腹部的病变组织及其鳃、心肌、肝胰腺、生殖腺等器官和组织 ,经光镜和电镜制样观察 ,未发现病原体 ,表明该病为非病原体引起的疾病。光镜下的病变组织结构与正常组织相比无明显异常。但在电镜下 ,病变主要表现为肌肉细胞的线粒体和肌质网的变性、坏死。线粒体肿胀、空泡化 ,肌质网增多且形成许多内陷小泡 ,表明细胞处于缺氧和钙代谢紊乱状态 ,这可能与虾生存的环境和养殖条件有关。根据超微病理与组织学观察结果 ,认为“罗氏沼虾白体病”更确切的命名应为“罗氏沼虾肌肉细胞线粒体坏死症”

Ultrastructural observation on muscle tissues of Macrobrachium rosenbergii with white-body disease

The light microscopy and electron microscopy were used to observe the pathological changes in Macrobrachium rosenbergii suffering from white body disease.The pathogen were not found in the pathological area abdominal muscle or other nonpathological areas such as gill, heart, hepaticopancreas and gonad. The results indicate that the disease is not caused by any pathogen. The histopathological observation shows that there is no obvious difference between normal and diseased tissues in the early and middle periods of the disease. But the ultrapathological observation shows obvious pathological process in the mitochondria and sarcoplasmic reticulum in abdominal muscle in those periods. The phenomenon of tumescent and disruptive mitochondria sometime with dense electronic particles and increased sarcoplasmic reticulum with many small bubbles indicate that the muscle cells are in the conditions of oxygen deficit and Ca 2 metabolic disorder, which maybe due to the deterioration of environment and cultural conditions. Based on the ultrastructural results,the white body disease of M.rosenbergii should be named as 'Muscle Cell Mitochondria Necrosis'.