Estimation of the period sensitive for the development of abnormal morphology in brown sole Pseudopleuronectes herzensteini fed live food enriched with docosahexaenoic acid |
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Authors: | Nobukazu Satoh Toshio Takeuchi |
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Affiliation: | (1) Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo 108-8477, Japan;(2) Present address: Hokkaido Mariculture Fisheries Experimental Station, Muroran Hokkaido, 051-0013, Japan |
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Abstract: | The aim of the present study was to clarify the effect of a feeding regimen of live food enriched with docosahexaenoic acid (DHA) on the rates of abnormal morphology in larval brown sole Pseudopleuronectes herzensteini. Live foods were enriched with oil emulsion to produce both high-DHA and low-DHA content. Larvae in group 1 (control) were fed low-DHA-content live foods throughout the experiment. Conversely, larvae in group 2 were fed high-DHA live foods from 15 days post-hatching (dph), when larvae were D stage. Larvae in group 3 were fed high-DHA live foods from 25 dph, when larvae consisted mainly of E stage. The occurrence of normal morphology development in group 2 was significantly higher (P < 0.05) than those of groups 1 and 3, suggesting that normal morphology of brown sole is determined during the critical larval period (D–E stages). |
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Keywords: | Brown sole Docosahexaenoic acid Live food Normal morphology Pseudoalbinism Sensitive period |
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