水稻黄叶突变体叶绿体超微结构与突变基因定位

对黄叶突变体-黄玉B的叶绿体超微结构和遗传特性进行研究。结果表明,野生型和突变体在相同的叶龄期,叶片内叶绿体个数差异不显著(P<0.05),但野生型的叶绿体基质浓厚、基粒片层堆叠比较整齐、有序,突变体基质较淡、基粒片层堆叠比较松散。遗传分析表明,该突变体黄叶性状受1对隐性基因控制,命名为xl(t);用SSR分子标记将xl(t)定位在第11染色体RM5349和RM21之间,遗传距离分别为0.82 cM和2.34 cM。

Gene mapping and chloroplast ultrastructure of a xantha rice mutant

The chloroplast ultrastructure and genetic characteristics of a xantha rice mutant were studied in this paper. The result showed that the chloroplast number in leaves of mutant was not significantly different from its wild parent (P<0.05). Compared to its wild type parent, chloroplasts of the mutant had thin stroma and disorderly stacked grana lamellae. Genetic analysis demonstrated that the xantha leaf mutation was controlled by a single recessive gene named as xl(t) and found to be located between RM5349 and RM21 on chromosome 11 with the genetic distance of 0.82 cM and 2.34 cM, respectively.