Field bean (Phaseolus vulgaris L.) cultivar identification by electrophoregrams of cotyledon storage proteins

Summary Electrophoretic procedures were developed for seed proteins which can discriminate cultivars of field beans. Proteins were extracted from seven varieties and the extracts were analysed using acid and SDS polyacrylamide gel electrophoresis. Electrophoregrams are presented to illustrate the results that can be obtained with the methods described. Results indicate that sufficient variation is present among the seven cultivars examined to afford unambiguous discrimation and identification of the cultivars. Banding patterns were stable for each genotype.Joint publication of the Department of Plant Science, University of Manitoba (No. 747) and Centro Internacional de Agricultural Tropical-CIAT.