Improvement of the identification of staphylococci isolated from bovine mammary infections using molecular methods |
| |
Authors: | Bes M Guérin-Faublée V Meugnier H Etienne J Freney J |
| |
Institution: | Centre National de Référence des Toxémies à Staphylocoques, UPRES EA 1655, Laboratoire de Bactériologie, Faculté de Médecine R.T.H. Laennec, Lyon, France. |
| |
Abstract: | Fifty-six Staphylococcus strains isolated from cases of bovine mammary infections were identified by using phenotypic and genotypic methods. Twenty-eight strains (50%) were identified at the species level according to their phenotypic characteristics, whereas the remaining 28 strains presented atypical or unreliable profiles. A combination of phenotypic and genotypic methods allowed the 56 strains studied to be classified. Internal transcribed spacer-polymerase chain reaction (ITS-PCR) based on the polymorphism of the 16S-23S rDNA spacer region appeared as a rapid and reliable method for the classification of bovine staphylococcal isolates at the species and subspecies levels. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|