| 油梨种质的离体低温保存 |
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| 引用本文： | 彭民璋,邓九生,甘霖.油梨种质的离体低温保存[J].果树学报,1996(2). |
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| 作者姓名： | 彭民璋 邓九生 甘霖 |
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| 作者单位： | 广西农垦职工大学，湖南农业大学 |
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| 摘 要： | 以哈斯油梨（ＰｅｒｓｅａａｍｅｒｉｃａｎａＭｉｌｌｃｖ．Ｈａｓｓ）为试材，从试管苗下胚轴诱导产生愈伤组织，研究了愈伤组织的离体低温保存技术。结果表明，采用８℃低温，愈伤组织能在ＭＳ＋１．０ｍｇ·１~(-１)２，４一Ｄ＋３％椰乳＋３％蔗糖固体培养基中保存６个月；在培养基中添加０．４ｍｇ·１~(-１)多效唑能减少继代次数，使保存期延长至８个月。
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| 关 键 词： | 油梨 愈伤组织 离体保存 低温 |
Preservation of Avocado in vitro Under Low Temperature |
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| Peng Minzhang and Deng Jiusheng, Gan Lin.Preservation of Avocado in vitro Under Low Temperature[J].Journal of Fruit Science,1996(2). |
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| Authors: | Peng Minzhang and Deng Jiusheng Gan Lin |
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| Abstract: | Calli derived from the hypocotyl of avocado (Persea americana Mill cv.‘Hass')were stored at 8℃invitro. The results indicated that the calli when cultured on MS medium + 1. 0mg·1￣(-1)2,4一D+ 3%coconutmilk + 3%sucrose and stored for 6 months still showed comparatively higher viability.The duration ofpreservation might be extended to 8 months by adding 0.40 mg·1￣(-1)paclobutrazol. |
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| Keywords: | Avocado Callus in vitro Low temperature preservation |
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