| 猪IGFBP2基因多态性及其与胴体、肉质性状的关联分析 |
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| 作者单位： | ;1.湖北省生猪健康养殖协同创新中心农业部动物遗传育种与繁殖教育部重点实验室农业部猪遗传育种重点实验室华中农业大学;2.湖北省畜禽育种中心 |
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| 摘 要： | 本研究旨在调查猪胰岛素样生长因子结合蛋白2基因(IGFBP2)潜在的变异与胴体性状和肉质性状之间的关系。IGFBP2是IGFBPs家族的一员,定位在猪15号染色体上。本研究利用PCR-MspⅠ-RFLP方法对该基因第二内含子g.171CT(Gen Bank Accession No.BV727778)进行了单核苷酸多态性(SNP)检测,在382头大白×梅山猪F2代猪群体中关联分析。结果发现该位点与背膘厚、眼肌高、眼肌宽、骨率等性状呈极显著相关(P0.01),与眼肌面积、肥肉率、瘦肥比、花油、色值(LD)和色值(BF)等性状呈显著相关(P0.05)。结果表明IGFBP2基因型与胴体和肉质性能存在一定程度的显著关联,是潜在的影响猪胴体和肉质性状的候选基因。
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| 关 键 词： | IGFBP2 多态性 胴体性状 肉质性状 关联分析 猪 |
Polymorphism of IGFBP2 Gene and Its Association with Carcass and Meat Quality Traits in Pigs |
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| Institution: | ,The Cooperative Innovation Center for Sustainable Pig Production of Hubei Province, Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture & Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University,Livestock and Poultry Breeding Center of Hubei Province |
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| Abstract: | This study was conducted to investigate the potential association of variation in the insulin-like growth factor binding protein 2(IGFBP2) gene with carcass and meat quality traits in pigs. IGFBP2 is a member of IGFBP family, maps to chromosome 15. PCR-Msp Ⅰ- RFLP method was used to detect the SNP g.171C>T(Gen Bank Accession No. BV727778) at the second intron in Large White × Mei Shan F2 population(n=382). This SNP was significantly associated with back fat thickness, loin eye height, eye muscle width, bone percentage, and other traits(P <0.01), and was significantly associated with eye muscle area, fat percentage and the ratios of fat to lean, flower fat, color value(LD) and color value(BF)(P <0.05). These results suggested that IGFBP2 as a potential candidate gene affects on carcass and meat traits. |
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| Keywords: | IGFBP2 Polymorphism Carcass traits Meat quality traits Association analysis Pig |
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