Low temperature-induced water-soaking of Dendrobium inflorescences: Relation with phospholipase D activity,thiobarbaturic-acid-staining membrane degradation products,and membrane fatty acid composition |
| |
| Institution: | 1. Department of Horticulture, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand;2. Postharvest Technology Innovation Center, Commission on Higher Education, Bangkok 10400, Thailand;3. Academy of Science, The Royal Institute, Dusit, Bangkok 10300, Thailand;4. Mann Laboratory, Department of Plant Sciences, University of California, Davis, CA 95616, USA;1. College of Chemistry, Key Lab of Environment Friendly Chemistry and Application in Ministry of Education, Xiangtan University, Xiangtan 411105, China;2. State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, China;1. Department of Plant Agriculture, University of Guelph, 50 Stone Rd. E., Guelph, ON N1G 2W1, Canada;2. Department of Mathematics and Statistics, University of Guelph, 50 Stone Rd. E., Guelph, ON N1G 2W1, Canada;3. Ontario Ministry of Agriculture and Food, Box 587, 1283 Blueline Rd. at Highway 3, Simcoe, ON N3Y 4N5, Canada;1. Horticulture and Product Physiology Group, Dept. of Plant Sciences, Wageningen University, P.O. Box 16, 6700 AA Wageningen, The Netherlands;2. Department of Horticulture, College of Aburaihan, University of Tehran, 3391653775, Pakdasht, Tehran, Iran;1. College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, PR China;2. Guangdong Provincial Engineering Technology Research Center for Protected Horticulture, College of Horticulture, South China Agricultural University, Guangzhou 510642, PR China |
| |
| Abstract: | We compared the effects of cold storage (5 °C) on two Dendrobium cultivars. After various periods of storage the cut inflorescences were transferred to 25 °C. Water-soaking of the tepals was the main chilling-injury symptom. Cv. Earsakul showed higher CI sensitivity than cv. Khao Sanan. Phospholipase D (PLD) activity increased during 5 °C storage in both cultivars, but generally increased more in cv. Earsakul during the subsequent period at 25 °C. The level of lipid degradation products (including malondialdehyde) that react with thiobarbaturic acid was higher in cv. Earsakul than in cv. Khao Sanan. In contrast to expectation, the ratio between unsaturated and saturated fatty acids was higher, and remained higher during cold storage, in cv. Earsakul than in cv. Khao Sanan. The data suggest that water-soaking due to cold storage of Dendrodium inflorescences involves membrane damage, likely related to activation of PLD and accumulation of lipid degradation products. The level of unsaturated fatty acids apparently did not protect against CI. The data seem to question the general view that unsaturated fatty acids protect against CI. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
