Overexpression of an nsLTPs-like antimicrobial protein gene (LJAMP2) from motherwort (Leonurus japonicus) enhances resistance to Sclerotinia sclerotiorum in oilseed rape (Brassica napus) |
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| Institution: | 1. State Key Laboratory of Eco-Environment and Bio-Resource of Three Gorges Reservoir Region, Key Laboratory of Eco-Environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing 400715, China;2. Key Laboratory of Biotechnology and Crop Quality Improvement of Ministry of Agriculture of China, Biotechnology Research Center, Southwest University, Chongqing 400715, China;1. Creative Chemistry and Innovation Research Unit, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahasarakham University, Mahasarakham 44150, Thailand;2. Materials Chemistry Research Center, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand;3. Department of Chemistry, Faculty of Engineering, Rajamangala University of Technology Isan, Khon Kaen Campus, Khon Kaen 40000, Thailand;1. Department of Anesthesiology and Perioperative Care, University of California Irvine, 101 South City Drive, Orange, CA 92868, USA;2. Department of Anesthesiology and Perioperative Care, Erasme University Hospital, Free University of Brussels, 808 Route de Lennick, 1070 Brussels, Belgium;1. Department of Plant & Microbial Biology, University of Minnesota, 1479 Gortner Ave, St. Paul, MN 55108, USA;2. Biology Department, St. Catherine University, 2004 Randolph Ave, St. Paul, MN 55105, USA;1. School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, China;2. School of Life Sciences and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, China;3. Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China;1. College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;2. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;3. Life science Faculty, Kim Il Sung University, Pyongyang 999093, Democratic People''s Republic of Korea |
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| Abstract: | Sclerotinia stem rot caused by Sclerotinia sclerotiorum is one of the most important diseases of oilseed rape worldwide and leads to considerable yield losses. In this study, a non-specific lipid transfer protein-like antimicrobial protein gene (LJAMP2) from motherwort (Leonurus japonicus) was introduced into oilseed rape (Zhongyou 821) by Agrobacterium-mediated transformation. In vitro experiments revealed that the mycelial growth of S. sclerotiorum was significantly inhibited when supplied with crude leaf extracts from transgenic oilseed rape plants overexpressing LJAMP2. Furthermore, in vivo studies showed that transgenic LJAMP2 plants had enhanced resistance to S. sclerotiorum. Semi-quantitative RT-PCR analysis showed that the LJAMP2 gene was transcribed in all transformed plants. In addition, we also found that overexpression of LJAMP2 in transgenic plants caused constitutive activation of the defense-related gene PR-1 and an increase of H2O2 production, but did not enhance PDF1.2 expression. Our results suggest that constitutive expression of the LJAMP2 gene from motherwort seeds might be exploited to improve the resistance of oilseed rape against S. sclerotiorum. |
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