Overexpression of the ethylene response factor SlERF1 gene enhances resistance of tomato fruit to Rhizopus nigricans |
| |
| Affiliation: | 1. College of Life and Food Engineering, Chongqing Three Gorges University, Chongqing, 404000, PR China;2. College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, PR China;3. College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, 315211, PR China;1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013 Jiangsu, People’s Republic of China;2. School of Grain Science and Technology, Jiangsu University of Science and Technology, Zhenjiang, 212003, People’s Republic of China;1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei, 430070, China;2. Hubei Hongshan Laboratory, Wuhan, China;1. Key Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing University, Chongqing, 401331, China;2. Center of Plant Functional Genomics, Institute of Advanced Interdisciplinary Studies, Chongqing University, 401331, Chongqing, China;3. School of Life Sciences, Chongqing Normal University, Chongqing, 401331, China;1. School of Life Sciences, Chongqing Normal University, Chongqing 401331, China;2. Key Laboratory of Plant Hormones and Development Regulation of Chongqing, School of Life Sciences, Chongqing University, Chongqing 401331, China |
| |
| Abstract: | The tomato SlERF1 gene belongs to a distinct subfamily of the large ERF gene family. It is a downstream component in the ethylene signaling pathway in tomato, and is involved in plant defense and stress responses. Overexpression of the SlERF1 gene (SlERF1-OEs) in tomato dramatically enhanced the resistance of fruit to Rhizopus nigricans. In attempt to elucidate the mechanism basis of this observation, our study revealed a dual modulation. Firstly, SlERF1-OEs fruit showed a remarkable transcript accumulation of PR5 and PAL genes before infection with R. nigricans, which was not observed in wild-type (WT) fruit. Secondly, SlERF1-OEs fruit had higher activitiers of phenylalanine ammonia lyase (PAL) and chitinase (CHI) in response to the fungal stress. These results show that SlERF1 positively modulated the ethylene-dependent pathogenesis defense pathway in tomato. That may be one of the mechanisms by which ERFs enhance plant tolerance to fungal invasion. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
