Molecular and functional characterization of bacteria isolated from straw and goat manure based vermicompost |
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| Institution: | 1. Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar 143005, India;2. P.G. Department of Zoology, Khalsa College, Amritsar 143001, India;3. Solid and Hazardous Waste Management Division, National Environmental Engineering Research Institute (CSIR-NEERI), Nagpur 440020, India;1. Department of Biotechnology, Aarupadai Veedu Institute of Technology, Vinayaka Missions Research Foundation (Deemed to be University), Paiyanoor, Chennai 603 104, Tamil Nadu, India;2. Department of Botany, Annamalai University, Annamalai Nagar 608 002, Tamil Nadu, India;3. Vermitechnology and Ecotoxicology Laboratory, Department of Zoology, School of Life Sciences, Periyar University, Salem 636 011, Tamil Nadu, India;4. Department of Biotechnology, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences, Saveetha Nagar, Chennai 602 105, Tamil Nadu, India;5. Department of Environmental Energy and Engineering, Kyonggi University, Youngtong-Gu, Gyeonggi-Do 16227, South Korea;6. Department of Botany, Government Arts College (Autonomous), Salem 636 007, Tamil Nadu, India;1. Institute of Soil Biology, Biology Centre CAS, Na Sádkách 7, CZ-37005 ?eské Budějovice, Czech Republic;2. Faculty of Science, University of South Bohemia, Brani?ovská 31, CZ-37005 ?eské Budějovice, Czech Republic;3. Department of Soil and Water Conservation and Organic Waste Management, Centro de Edafología y Biología Aplicada del Segura, CSIC, P.O. Box 4195, E-30080 Murcia, Spain;1. Animal Environment Division, Department of Animal Biotechnology and Environment, National Institute of Animal Science, RDA, Jeonju, South Korea;2. Sino-Forest Applied Research Centre for Pearl River Delta Environment, and Department of Biology, Hong Kong Baptist University, Hong Kong Special Administrative Region;3. Environmental Technology Division, Central Leather Research Institute, Chennai, India;1. Departamento de Ecoloxía e Bioloxía Animal, Universidade de Vigo, Vigo, E-36310. Spain;2. CIBIO-InBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Campus Agrário de Vairão, 4485-661 Vairão, Portugal;3. Computational Biology Institute, George Washington University, Ashburn, VA 20147, USA;4. Department of Invertebrate Zoology, US National Museum of Natural History, Smithsonian Institution, Washington, DC 20013, USA |
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| Abstract: | A total of 193 vermicompost bacteria that exhibit antagonistic and biofertilizing potential were isolated from straw and goat manure based vermicompost produced by earthworm Eisenia foetida and taxonomically designated on the basis of their 16S rRNA sequence homology and subsequent molecular phylogeny analysis. Bacteria belonged to three major genera viz., Pseudomonas (15%), Bacillus (57%), Microbacterium (12%) and remaining bacteria comprised of genus Acinetobacter (5%), Chryseobacterium (3%), Arthrobacter, Pseudoxanthomonas, Stenotrophomonas, Paenibacillus, Rhodococcus, Enterobacter, Rheinheimera and Cellulomonas. Of 193 bacteria, 96 strains (49%) showed antagonistic potential against phytopathogenic fungus. Functional characterization of the bacteria was assessed by the production of protease, cellulase, lipase, xylanase, chitinase, amylase, gelatinase, 1-aminocyclopropane-1-carboxylate (ACC) deaminase, indole-3-acetic acid (IAA), phosphate solubilization, nitrate reduction, and assimilation of different carbon sources. The bacterial strains showed a varying degree of carbon utilization profiles and majority of them were known to utilize citrate, malonate. A total of 86 strains produced protease (44%) and 99 strains (51%) produced cellulase. 51 strains (26%) produced IAA, 99 strains (51%) produced siderophores and 124 strains (64%) produced ACC deaminase. 52 strains (27%) showed phosphate solubilization while 24 strains (12%) produced HCN. 43 strains (22%) showed antibacterial activity against human pathogens under in vitro conditions. None of the strains tested positive for lipase and chitinase. A total of 31 strains (16%) produced DNase, 80 strains (41%) produced xylanase and 93 strains (48%) produced amylase. Among the vermicompost bacteria reported in the present study, Bacillus was found to be the predominant bacteria followed by Pseudomonas and Microbacterium. Present study, reports the molecular and functional characterization of vermicompost bacteria. |
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| Keywords: | Vermicompost bacteria 16S rRNA diversity Plant growth-promotion Antagonism Waste management |
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