Pre-harvest nitrogen and Azoxystrobin application enhances postharvest shelf-life in Butterhead lettuce

Two trials were carried out on Butterhead lettuce (March–May 2008 and April–June 2009) to investigate the effect of the application of nitrogen fertilizer (0, 50 and 100 kg ha^?1 of N) and of strobilurin (Azoxystrobin, methyl (E)-2-{2-6-(2-cyanophenoxy)pyrimidin-4-yloxy]phenyl}-3-methoxyacrylate) on (i) yield and morphological traits at harvest, (ii) physical (weight loss and dry matter), visual (chlorophyll content and main colour indices), physiological (relative water content, osmotic potential, and electrolytic leakage), and nutritional (ascorbic acid, nitrate, and polyphenol content) quality of raw material and their changes after storage of fresh-cut leaves. Cool storage lasted 7 and 12 days in the first and second experiment, respectively. In the first cycle, under early-spring conditions, lettuce yield was lower by 38% and, even if the product was lighter coloured higher L* (+6%) and lower CHL (?21%)], it had lower dry matter content (?32%), higher electrolyte leakage (EL) (+14%) and WL_Trans (+8%) compared with the raw product from the second cycle. In both years, the increase of N supply and the application of Azoxystrobin improved yield (by 8.5% and 10%, respectively). The response in N fertilization was more evident under early-spring (2008) compared to late-spring (2009) conditions (12.3% vs. 4.8%), and when (2008) the highest N rate interacted with the application of Azoxystrobin (+12.9% compared with the other treatments). The nitrate content in leaves was always reduced by Azoxystrobin application (?43%) and increased with the N supply (+53%). In the second experiment, when storage was prolonged for 12 days, strobilurin improved postharvest shelf-life by reducing chlorophyll degradation (?27%), senescence (?19%, measured as EL), and browning (?53%, measured as h° index decrease). Azoxystrobin lowered also the total polyphenol content of raw material (?12.5%), which can be linked to less browning during storage. During postharvest storage, irrespective to the preharvest dose, N supply kept the visual quality and physiological senescence indices constant (L*, h° and EL). The suitability of the Butterhead lettuce to fresh-cut processing depends on climatic growing conditions. Preharvest Azoxystrobin supply improves the nutritional quality of the raw material, reducing leaf nitrate content, and the shelf-life in prolonged storage. The N rate of 100 kg ha^?1 of N is suitable under less favourable growing conditions, while the rate of 50 kg ha^?1 is better for more favourable climatic conditions, especially if a moderate contribution in available N from soil organic matter mineralization and no leaching from heavy rains is expected.