| 家鸡微卫星引物在灰胸竹鸡中的跨种扩增和特征分析 |
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| 引用本文： | 黄族豪,廖信军. 家鸡微卫星引物在灰胸竹鸡中的跨种扩增和特征分析[J]. 中国鸟类, 2010, 1(1): 74-76. DOI: 10.5122/cbirds.2009.0002 |
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| 作者姓名： | 黄族豪 廖信军 |
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| 作者单位： | 井冈山大学生命科学学院,江西吉安,343009 |
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| 摘 要： | 灰胸竹鸡(Bambusicola thoracica)是中国特有鸟类.利用近缘种家鸡(Gallus gallus)中已发表的微卫星DNA标记引物,对灰胸竹鸡进行跨种扩增,得到10个多态位点.每个位点的等位基因数为4-13个,观察杂合度为0.1220-1.000,期望杂合度为0.1183-0.8898.4对引物显著偏离Hardy-Weinberg平衡.这些引物将为研究灰胸竹鸡的系统地理学和保护遗传学提供非常有用的工具.
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| 关 键 词： | 灰胸竹鸡 微卫星 多态性 |
Cross-species amplification and characterization of microsatellite DNA loci from Gallus gallus in Bambusicola thoracica |
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| Zuhao HUANG,Xinjun LIAO. Cross-species amplification and characterization of microsatellite DNA loci from Gallus gallus in Bambusicola thoracica[J]. Chinese Birds, 2010, 1(1): 74-76. DOI: 10.5122/cbirds.2009.0002 |
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| Authors: | Zuhao HUANG Xinjun LIAO |
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| Abstract: | The Chinese Bamboo Partridge(Bambusicola thoracica)is a gamebird endemic to China.Ten polymorphic microsatellite(simple sequence repeat)markers were obtained through cross-species amplification for this partridge from its relative species Gallus gallus.The number of alleles per locus varied from 4 to 13.The observed heterozygosity ranged from 0.1220 to1.0000 and the expected heterozygosity from 0.1183 to 0.8898.Four microsatellite loci showed significant differences from the Hardy-Weinberg equilibrium.These polymorphic loci provide a valuable tool for the investigation of the phylogeography and conservation genetics of this partridge. |
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| Keywords: | Bambusicola thoracica microsatellite loci polymorphism |
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