枸骨内生真菌抗菌代谢产物的鉴定及活性研究

对一株枸骨内生真菌哈茨木霉Trichoderma harzianum抗菌活性成分的分离、鉴定及其抗菌生物活性进行了研究。该真菌发酵液经乙酸乙酯萃取、正相硅胶和ODS-反相色谱分离纯化得到一单端孢霉烯化合物,经红外、质谱和核磁分析,鉴定为:4β-乙酰基-12,13-环氧-9-单端孢霉烯(trichodermin),这是首次从哈茨木霉菌代谢产物中分离出该化合物。Trichodermin对蕃茄早疫病和黄瓜立枯病病原菌的离体抑制活性EC50值分别为3.35和3.59 mg/L;活体测定结果表明,在 100 mg/L的剂量下对两种病的保护效果分别为97.8% 和98.1%,治疗效果为96.7%和97.3%。

Identification and Antifungal Activity of the Metabolite of Endophytic Fungi Isolated from Llex cornuta

The isolation,identification and antifungal activity of the active metabolite of the endophytic fungi Trichoderma harzianum from Llex cornuta Lindl.were studied.The fermentation liquor of the endophytic fungi was extracted by ethyl acetate and purified by silica gel and reversed phase chromatography(ODS).A trichothecene compound was obtained and its structure was identified as 4β-acetoxy-12,13-epoxy-9-trichothecene(trichodermin) by IR,MS and NMR.The compound was separated from the metabolite of Endophytic Fungi for the first time.The EC50 values of trichodermin inhibiting the mycelia growth of Alternatia solani and Rhizoctonia solani were 3.35 and 3.59 mg/L,respectively.In addition,protective effects and therapeutic effects of trichodermin at 100 mg/L against A.solani and R.solani were 97.8%,98.1% and 96.7%,97.3%,respectively.