Increasing erucic acid content in Ethiopian mustard through mutation breeding |
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Authors: | L. VELASCO,J. M. FERNÁ NDEZ-MARTINEZ,A. de,HARO |
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Affiliation: | Instituto de Agricultura Sostenible (CSIC), Apartado 4084, E-14080 Córdoba, Spain |
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Abstract: | Ethylmethane sulphonate (EMS) was applied to seeds of the Ethiopian mustard (Brassica carinata A. Braun) line C-101. Bulk samples of M3 seeds from 8331 M2 plants were evaluated for the fatty acid composition of their oil by near-infrared reflectance spectroscopy (NIRS) and by further gas chromatography on selected samples. A putative mutant, N2-6230, showing very low oleic acid content (4.7% vs. average of 8.6% in C-101) and erucic acid content within the range of variation of the line C-101 (40-49.3%) was identified. The M3 progeny of this mutant showed a wide segregation for erucic acid content (39.1-57.9% vs. 41.8-50.3% in C-101), and maintained levels of oleic acid lower than in line C-101. Selection for high erucic acid content in the M3 and M4 generations led to the fixation of this mutation in the M5 generation (52.2-59.3% vs. 39.0-47.6% in C-101). This is the first high erucic acid line obtained in Brassica species through mutation breeding. Its utility in future programmes to develop very high erucic acid lines is discussed. |
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Keywords: | Brassica carinata Ethiopian mustard high erucic acid content mutation breeding ethylmethane sulfonate near-infrared reflectance spectroscopy oil quality |
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