Simplified application of return gel electrophoresis for the routine detection of potato spindle tuber viroid |
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Authors: | M SCHROEDER H-L WEIDEMANN |
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Institution: | Biologische Bundesanstalt für Land- und Forstwirschaft, Messeweg 11/12, 3300 Braunschweig (FRG) |
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Abstract: | The return gel electrophoresis method for the detection of potato spindle tuber viroid has been modified to simplify its use for large-scale testing. The quantities of dissolvents for the extraction of nucleic acids were reduced and adapted to the use of disposable plastic tubes. The vertical electrophoresis system was replaced by a horizontal one, which was easier to handle. Migration distance for the separation of PSTV bands was shortened, saving time for the electrophoretic run. A single-buffer system was used. The detection limit was estimated as 0.3 ng per slot. It was possible to detect both mild and severe strains of PSTV. |
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