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| 菠萝种质鉴定及亲缘关系的AFLP分析 | |
| 引用本文： | 刘卫国,易干军,刘岩,张秋明,曾继吾. 菠萝种质鉴定及亲缘关系的AFLP分析[J]. 果树学报, 2008, 25(4) |
| 作者姓名： | 刘卫国 易干军 刘岩 张秋明 曾继吾 |
| 作者单位： | 1. 吉首大学城乡资源与规划学院,湖南张家界,427000;湖南农业大学园艺园林学院,长沙,410128;广东省农业科学院果树研究所,广州,510640 2. 广东省农业科学院果树研究所,广州,510640 3. 湖南农业大学园艺园林学院,长沙,410128 |
| 基金项目： | 广东省科技厅农业科技攻关项目 |
| 摘 要： | 从FISH-AFLP试剂盒(PSTI/MseI型)64对引物中,筛选出8对AFLP选择性引物P-GAA/M-CTA、P-GAC/M-CTG、P-GAC/M-CTT、P-GAG/M-CAT、P-GAG/M-CTA、P-GAT/M-CAT、P-GAT/M-CTA、P-GAT/M-CTC,对39份菠萝种质进行了AFLP分析,都得到了清晰的多态性指纹图谱。8对引物共获得454个遗传位点,其中多态性位点332个,多态性比例平均为73.1%。UPGMA聚类表明,各种质间的相似系数为0.73～0.98,说明39份菠萝种质遗传关系相对较近。依相似系数0.80的水平,将供试的39份菠萝种质分为4类。研究结果可以作为菠萝分类的理论依据和基础。 |
| 关 键 词： | 菠萝 亲缘关系 AFLP |
Identification of pineapple germplasm and relationship analysis with AFLP |
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| LIU Wei-guo,YI Gan-jun,LIU yan,ZHANG Qiu-ming,ZENG Ji-wu. Identification of pineapple germplasm and relationship analysis with AFLP[J]. Journal of Fruit Science, 2008, 25(4) | |
| Authors: | LIU Wei-guo YI Gan-jun LIU yan ZHANG Qiu-ming ZENG Ji-wu |
| Abstract: | Eight pairs of primers, including P-GAA/M-CTA, P-GAC/M-CTG, P-GAC/M-CTT, P-GAG/M-CAT, P-GAG/M-CTA, P-GAT/M-CAT, P-GAT/M-CTA and P-GAT/M-CTC were selected for AFLP analysis from the 64 primer pairs supplied with FISH-AFLP kit. A total of 454 loci were amplified from 39 pineapple accessions, among which, 332 (73.1%) were polymorphic. UPGMA clustering analysis indicated that the Dice’s coefficient of the 39 pineapple accessions ranged from 0.73 to 0.98 indicating relatively close relationship among these accessions. Based on the results, the 39 accessions could be divided into 4 categories at a Dice coefficient value of 0.80. The results provided theoretical basis for the classification of pineapple. |
| Keywords: | Pineapple Relationship AFLP |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |