| 3-氨基丙基三乙氧基硅烷改性凹凸棒土固定化菠萝蛋白酶工艺研究 |
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| 引用本文： | 蒋荣,单良,王兴国.3-氨基丙基三乙氧基硅烷改性凹凸棒土固定化菠萝蛋白酶工艺研究[J].安徽农业科学,2008,36(25). |
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| 作者姓名： | 蒋荣 单良 王兴国 |
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| 作者单位： | 食品科学与技术国家重点实验室,江南大学食品学院,江苏无锡,214122;食品科学与技术国家重点实验室,江南大学食品学院,江苏无锡,214122;食品科学与技术国家重点实验室,江南大学食品学院,江苏无锡,214122 |
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| 基金项目： | 江南大学食品学院本科生课余研究项目 |
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| 摘 要： | 目的]确定利用3-氨基丙基三乙氧基硅烷改性凹凸棒土(3-APTS-凹凸棒土)固定菠萝蛋白酶的最佳工艺条件。方法]以3-APTS-凹凸棒土为基质,在单因素试验的基础上,研究用固定菠萝蛋白酶的最佳工艺条件。结果]确定菠萝蛋白酶固定化的条件为:0.4%戊二醛交联,温度38~44℃,pH值6.4,固定化时间4.5 h,最终产品的酶活为3 900 U/g,酶活回收率达80%。结论]利用3-APTS-凹凸棒土固定的菠萝蛋白酶稳定性和利用率显著提高。
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| 关 键 词： | 菠萝蛋白酶 3-氨基丙基三乙氧基硅烷改性凹凸棒土 固定化 |
Study on the Immobilization Technology of Bromelin with Attapulgite modified by 3-aminopropyltriethoxysilane |
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| JIANG Rong et al.Study on the Immobilization Technology of Bromelin with Attapulgite modified by 3-aminopropyltriethoxysilane[J].Journal of Anhui Agricultural Sciences,2008,36(25). |
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| Authors: | JIANG Rong |
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| Abstract: | Objective] The research aimed to optimize the optimum technology conditions of immobilizing bromelin with attapulgite modified by 3-aminopropyltriethoxysilane.Method] Using 3-APTS-attapulgite as matrix,based on single factor test,the optimum technology conditions for immobilizing bromelin were studied.Result] The immobilization conditions of bromelin were confirmed as follows: crosslinking with 0.4% glutaraldehyde at 38-44 ℃,pH 6.4,immobilization time of 4.5 h.The enzyme activity of the final products was 3 900 U/g and the enzyme recovery reached 80%.Conclusion] The stability and utilization rate of bromelin immobilized with 3-APTS-attapulgite were significantly improved. |
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| Keywords: | Bromelin Attapulgite modified by 3-aminopropyltriethoxysilane Immobilization |
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