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Cytological analysis of plant chromosomes using rapid in situ hybridization
Authors:S M Reader  T E Miller  K A Purdie
Institution:(1) John Innes Centre, Colney, NR4 7UH Norwich, U.K.
Abstract:Summary A rapid fluorescent in situ hybridization protocol, which can be completed in one working day, has been utilised to visualize chromosome preparations of barley, chickpea, white clover, millet, rye, sugarcane, wheat, Aegilops spp. and Leymus spp. Both total genomic DNA and cloned nuclear rDNA were used as a probe. This protocol has the advantage of brevity and flexibility over more conventional approaches, and has made fluorescent in situ hybridization a routine cytological procedure.
Keywords:rapid fluorescent in situ hybridization  pre-annealing  barley (Hordeum vulgare)  chickpea (Cicer reticulatum)  white clover (Trifolium repens)  millet (Pennisetum glaucum)  rye (Secale cereale)  sugarcane (Saccharum officinarum)  wheat (Triticum aestivum)  Aegilops spp    Leymus spp
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