| 蒽酮—硫酸法测定桑葚乌发粥中粗多糖的含量 |
| |
| 引用本文： | 惠秋沙. 蒽酮—硫酸法测定桑葚乌发粥中粗多糖的含量[J]. 农产品加工．学刊, 2011, 0(10): 105-107 |
| |
| 作者姓名： | 惠秋沙 |
| |
| 作者单位： | 山东中医药大学 |
| |
| 摘 要： | 采用蒽酮—硫酸法,以葡萄糖为对照品,于波长625 nm处测其吸光度,测定总多糖的含量。葡萄糖质量浓度在0.02～0.14 mg/mL内与吸收度呈良好的线性关系;平均回收率为100.02%,RSD=0.116 6%;测得总多糖平均质量分数为16.26 mg/g。采用蒽酮—硫酸法测桑葚乌发粥中总多糖含量,快速准确,稳定性好,可作为该制剂总多糖含量的测定方法。
|
| 关 键 词： | 桑葚乌发粥 蒽酮硫酸法 多糖含量测定 |
Content Determination of Total Polysaccharides in Sangshen Wufa Gruel by Anthrone-sulfuric Acid Method |
| |
| Hui Qiusha. Content Determination of Total Polysaccharides in Sangshen Wufa Gruel by Anthrone-sulfuric Acid Method[J]. Nongchanpin Jlagong.Xuekan, 2011, 0(10): 105-107 |
| |
| Authors: | Hui Qiusha |
| |
| Affiliation: | Hui Qiusha(Shandong University of Traditional Chinese Medicine,Jinan,Shandong 250355,China) |
| |
| Abstract: | The content of total polysaccharides is measured colorimetrically by the anthrone-sulfuric acid method using glucose as the standard in the 625 nm wavelength.The calibration curve is linear within the range of 0.02～0.14 mg/mL for glucose.The average recovery is 100.02% with RSD 0.116 6%.The average content of polysaccharide is 16.26 mg/g.The method is simple,accurate and stable,which could be used for determining the contents of total polysaccharide in Sangshen Wufa gruel. |
| |
| Keywords: | Sangshen Wufa gruel Anthrone sulfuric acid method content determination of total Polysaccharide |
| 本文献已被 CNKI 等数据库收录！ |
|
