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| DNA分子标记在茶树种质资源鉴定中的应用 | |
| 引用本文： | 唐玉海,郭春芳,游小妹,陈常颂,张木清. DNA分子标记在茶树种质资源鉴定中的应用[J]. 茶叶科学技术, 2007, 0(1): 15-18 |
| 作者姓名： | 唐玉海 郭春芳 游小妹 陈常颂 张木清 |
| 作者单位： | 1. 农业部甘蔗生理生态与遗传改良重点开放实验室,福建福州,350002 2. 农业部甘蔗生理生态与遗传改良重点开放实验室,福建福州,350002;福建教育学院,福建福州,350002 3. 福建省农业科学院茶叶研究所,福建福安,355015 |
| 摘 要： | 分子标记具有多态性高、检测手段简单迅速、无基因多效性、能够明确辨别等位基因、实验重复性好等优点。目前最常用的分子标记技术主要有限制性片段长度多态性技术（restriction fragment length polymorphism，简称RFLP）、随机扩增多态性DNA技术（randomom amplified polymorphic DNA，简称RAPD），微卫星DNA技术（inter—Simple Sequence Repeat，简称ISSR）、扩增片段长度多态性技术（Amplified Fragment Length Polymorphism，简称AFLP）等。分子标记技术在茶树研究中得到了广泛的应用，在茶树遗传多样性、种质资源保护、遗传图谱的构建、基因定位与辅助选择育种、指纹图谱应用于作物品种鉴定等研究方面均取得较好的应用效果。 |
| 关 键 词： | DNA分子标记 种质资源鉴定 随机扩增多态性DNA技术 限制性片段长度多态性 应用 茶树 分子标记技术 扩增片段长度多态性 |
Application of Molecular Marking Technology to identification of tea germplasm |
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| Tang-Yuhai, Guo-Chunfang. Application of Molecular Marking Technology to identification of tea germplasm[J]. Tea Science and Technology, 2007, 0(1): 15-18 | |
| Authors: | Tang-Yuhai Guo-Chunfang |
| Affiliation: | Tang-Yuhai, Guo-Chunfang (1.Key Laboratory of Eco-physiology and Genetic Improvement for Sugarcane, Ministry of agriculture, Fuzhou 350002; 2.Fujian Education College, Fuzhou 350001) |
| Abstract: | DNA molecular marker technology directly works on DNA with the advantages of no restriction on plant develop stage, covering of all genome, allelic identification with numerous polymorphisms, simple and quick detection, and well experimental repeatability. Currently, there are four major DNA-based molecular mapping methods, RFLP, RAPD, AFLP and ISSR. These technologies are being widely applied in the areas of germplasm classification and reservation, genetic mapping, marker-assistant breeding, variety fingerprinting and proprietary rights protection. |
| Keywords: | RFLP RAPD AFLP ISSR Molecular Marker Tea |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |