Genetic 'Fingerprinting' of Sunflower Lines and F1 Hybrids using. Isozymes, Simple and Repetitive Sequences as Hybridization Probes, and Random Primers for PCR |
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| Authors: | G Mosges W Friedtu |
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| Institution: | Institut für Pflanzenbau und Pflanzenzüchtung I, Justus-Liebig-Universität, Ludwigstr. 23, D-35390 Gießen, Germany |
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| Abstract: | Three different marker systems which have been widely described in the characterization of genotypes were compared. Sunflower lines and hybrids were analysed by three isozymes, oligonucleotides (GACA)4, (GATA)4, and (GGAT)4 as hybridization probes, and 70 primers for PCR analysis. Polymorphisms were visible with each type of marker. However, the repeated probes, especially (GATA)4, revealed the highest degree of variability and seem to be most appropriate for the generation of genotype-specific ‘fingerprints.’ |
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| Keywords: | Heliantbus annuus isozymes DNA fingerprinting RAPD AP-PCR |
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