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平欧杂种榛过氧化物同工酶研究
引用本文:魏鑫,王玉霞,王淑敏,董文轩.平欧杂种榛过氧化物同工酶研究[J].山东农业科学,2008(1):22-25.
作者姓名:魏鑫  王玉霞  王淑敏  董文轩
作者单位:1. 沈阳农业大学园艺学院,辽宁,沈阳,110161;沈阳农业大学北方果树育种与生理生态研究所,辽宁,沈阳,110161
2. 沈阳农业大学园艺学院,辽宁,沈阳,110161
摘    要:本试验以62个平欧杂种榛品种(系)的新梢韧皮部为试材,利用聚丙烯酰胺垂直平板凝胶电泳,研究其过氧化物酶同工酶谱的特征。结果表明:平欧杂种榛韧皮部的过氧化物酶同工酶酶谱有3个区,共25条酶带,R f范围在0.087~0.557之间,多数品种(系)之间的谱带存在差异,部分品种(系)间酶带数量及强弱不同。62份资源的相似系数在0.5~1之间,距离系数在0~2.00之间。以距离系数1.6为标准,可将供试资源分为5大类。

关 键 词:过氧化物酶同工酶    聚丙烯酰胺凝胶电泳
文章编号:1001-4942(2008)01-0022-04
收稿时间:2007-11-02
修稿时间:2007年11月2日

Study of Peroxidase Isozyme of Ping'ou Hybrid Hazelnut ( Corylus L. )
WEI Xin,WANG Yu-xia,WANG Shu-min,DONG Wen-xuan.Study of Peroxidase Isozyme of Ping'ou Hybrid Hazelnut ( Corylus L. )[J].Shandong Agricultural Sciences,2008(1):22-25.
Authors:WEI Xin  WANG Yu-xia  WANG Shu-min  DONG Wen-xuan
Abstract:62 cultivars(strains) of Ping'ou hybrid hazelnut were used as experimental material.The characteristics of peroxidase isozymes extracted from phloem were studied by plumb plate polyacrylpropylene gel electrophoresis(PAGE).The results showed that there were three regions and 25 peroxidase isozyme belts totally in Ping'ou hybrid hazelnut,whose Rf ranged from 0.087 to 0.557.There were difference among belts of most cultivars(strains),and some of them were different in number and activity of belts.The similar coefficients of 62 resources were from 0.5 to 1 and the distance coefficient were from 0 to 2.00.62 resources could be divided into 5 groups by the distance coefficient of 1.6.
Keywords:Peroxidase isozyme  Hazelnut  PAGE
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