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Isolation and characterization of a Neisseria sp. from the captive wild goose (Anser anser)
Authors:Said AMER  Hua DING  Ying DONG  Hongxuan HE
Affiliation:1. National Research Center For Wildlife Born Diseases, Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China;2. Department of Zoology, Faculty of Science, Kafr El Sheikh University, Kafr El Sheikh, Egypt;3. Department of Infectious Diseases, Hangzhou Center for Disease Control and Prevention, Hangzhou, China;4. Department of Medical Oncology, the Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
Abstract:The present study investigated 15 dead cases of captive wild goslings (Anser anser), which were bred in a small poultry farm in Shandong Province, China. The examined cases presented diverse clinical signs accompanied with neurological manifestations and fatal outcomes. Bacterial culture identified the gram‐negative Neisseria sp. from the brain homogenate of most examined cases (10/15, 66.7%). The isolated bacteria were identified based on morphologic characteristics, biochemical tests and 16S rDNA typing. Results proved that 1 identical bacterial strain (BNO09‐3) was isolated from the positive cases. The phylogeny based on the 16S rDNA gene sequences indicated that this isolate has a close relationship with various strains of genus Neisseria sp. isolated from liver and feces of duck. This is the first report of Neisseria sp. causing fatality in captive wild geese in China.
Keywords:16S rDNA  Anser anser  China  Neisseria sp.
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