| 棉花凝集素的分离纯化及其对棉花枯萎病菌的影响 |
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| 引用本文： | 刘士庄,叶杨.棉花凝集素的分离纯化及其对棉花枯萎病菌的影响[J].植物病理学报,1996,26(4):311-315. |
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| 作者姓名： | 刘士庄 叶杨 |
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| 作者单位： | 上海师范大学生物系, 上海 200234 |
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| 基金项目： | 国家自然科学基金，上海自然科学基金 |
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| 摘 要： | 通过几丁质柱层析和Sephadex G-75过滤,得到SDS-PAGE电泳显一条蛋白带,专性结合甘露糖和葡萄糖,能凝集兔血红细胞的棉花凝集素,分子量为13400。利用电镜和凝集素探针检测棉花枯萎病菌分生孢子表面结构,发现分生孢子壁的骨架成份是几丁质,甘露糖和葡萄糖位于分生孢子壁外层,半乳糖被甘露糖和葡萄糖复盖。分子量为13,400的棉花凝集素能凝集枯萎病菌分生孢子,并抑制分生孢子萌发。
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| 关 键 词： | 棉花凝集素 棉花枯萎病菌 分生孢子 |
ISOLATION AND PURIFICATION OF COTTON LECTIN AND EFFECTION OF COTTON LECTIN TOFUSARIUM WILT PATHOGEN |
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| Liu Shizhuang,Shi Chengliang,Zhang Minjie,Ye Yang,Zhao Huifang,Cai Ma.ISOLATION AND PURIFICATION OF COTTON LECTIN AND EFFECTION OF COTTON LECTIN TOFUSARIUM WILT PATHOGEN[J].Acta Phytopathologica Sinica,1996,26(4):311-315. |
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| Authors: | Liu Shizhuang Shi Chengliang Zhang Minjie Ye Yang Zhao Huifang Cai Ma |
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| Institution: | Department of Biology, Shanghai Teacher's University, Shanghai 200234 |
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| Abstract: | By means of affinity chromatography and Shephadex G-75 filter, the cotton lectin was isolated and purified. The cotton lectin is able to specifically bind Mannose and Glucose. The cotton lectin is also able to agglutinate the conidiospores of Fusarium wilt pathogen and inhibit germ of the conidio spores of Fusarium wilt pathogen. |
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| Keywords: | Cotton lectin Fusarium wilt pathogen |
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