| 抑菌圈-定殖力双重测定法筛选青枯病生防细菌 |
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| 引用本文： | 郭坚华 王玉菊. 抑菌圈-定殖力双重测定法筛选青枯病生防细菌[J]. 植物病理学报, 1996, 26(1): 49-54 |
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| 作者姓名： | 郭坚华 王玉菊 |
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| 作者单位： | 南京农业大学植保系, 南京 210095 |
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| 摘 要： | 本研究首先用平皿抑菌圈法筛选出55个拮抗青枯菌的细菌菌株,将番茄幼苗在各菌菌悬液中浸根12h后栽种于温室未灭菌的土壤中,结果发现,有22个菌株在幼苗根部的定殖能力较强(终定殖密度大于104 cfu/g根),其中革兰氏阴性土壤细菌占同类菌的86.3%,革兰氏阳性土壤细菌占同类菌的13.0%,10个无致病力产细菌素的青枯菌菌株在根部的终定殖密度均低于104 cfu/g根,其定殖能力弱于致病青枯菌。有17个拮抗菌菌株在番茄幼苗根部的定殖密度超过所有致病菌。温室生防结果证明,抑菌圈-定殖力双重测定法对于筛选生防菌株是一种行之有效的方法。
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| 关 键 词： | 抑菌圈 定殖力 定殖密度 青枯假单胞杆菌 生物防治 |
SCREEN OF BIOCON TROL BACTERIA OF PLANT WILT BY INHIBITING ZONES AND ROOT-COLONIZING CAPACITY |
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| Guo Jianhua,Wang Yuju,Li Jin,Ren Xinzheng. SCREEN OF BIOCON TROL BACTERIA OF PLANT WILT BY INHIBITING ZONES AND ROOT-COLONIZING CAPACITY[J]. Acta Phytopathologica Sinica, 1996, 26(1): 49-54 |
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| Authors: | Guo Jianhua Wang Yuju Li Jin Ren Xinzheng |
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| Affiliation: | Dept. of Plant Protection, Nanjing Agric. Univ., Nanjing 210095 |
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| Abstract: | Fifty-five strains of Pseudomonas solanacearum inhibiting bacteria had been screened first by assessing the zones of inhibition on agar media. Dipped in each suspension of the strains for 12 hours, tomato seedlings were planted in raw soil under greenhouse conditions. |
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| Keywords: | Inhibiting zones Root colonizing capacity Root colonizing population Pseudomonas solanacearum Biocontrol |
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