| 用A蛋白免疫电镜快速检测几种蔬菜作物的两种杆状病毒 |
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| 引用本文： | 徐均焕 盛方镜. 用A蛋白免疫电镜快速检测几种蔬菜作物的两种杆状病毒[J]. 植物病理学报, 1996, 26(3): 231-236 |
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| 作者姓名： | 徐均焕 盛方镜 |
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| 作者单位： | 1. 浙江农业大学生物科技系, 杭州 310029;2. 浙江农业大学生物所, 杭州 310029 |
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| 摘 要： | 应用A蛋白免疫电镜技术对几种蔬菜作物的长叶车前草花叶病毒(RMV)、烟草花叶病毒(TMV)的检测比常规的电镜负染技术至少要灵敏近10,000倍,比普通的免疫电镜技术要灵敏10~100倍,抗血清浓度稀释至400,000倍,病毒粗提液稀释到1,000,000倍或病毒提纯液浓度为2.5×10-7 mg/ml时均能观察到病毒粒子。同时由于抗体分子能特异性地结合到病毒粒子表面,能够很容易地区分不同的病毒种类。
应用此项技术作者检测了杭州地区几种常见的蔬菜病毒病,对大白菜(Brassica campestris L.),蕃茄(Lycopersicum esculentum Mill),榨菜(Brassica juncea var.tsatsai Mao),雪里(Brassicajuncea var.multiceps Tsen et Lee)等作物发病期的叶或果的浸提液用A蛋白免疫电镜进行快速检测,结果在大白菜,番茄等病株上检测到较多的病毒粒子,且大多能被RMV抗血清修饰,同时在番茄病株检测到的少量病毒粒子亦能被TMV抗血清修饰,而榨菜及雪里等病株上检测到的病毒粒子很少能被RMV及TMV抗血清所修饰,另外,病毒粒子的含量以番茄病果中为最高,要比其它几种蔬菜作物叶子中高约10~30倍。
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| 关 键 词： | 蔬菜病毒病 RMV TMV 免疫电镜技术 |
| 收稿时间： | 1995-06-26 |
RAPID DETECTION OF TWO ROD-SHAPED VIRUSES OF SEVERAL VEGETABLE VIRUS DISEASES WITH PROTEIN A IMMUNOELECTRON MICROSCOPY |
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| Xu Junhuan,Cheng Fangjing. RAPID DETECTION OF TWO ROD-SHAPED VIRUSES OF SEVERAL VEGETABLE VIRUS DISEASES WITH PROTEIN A IMMUNOELECTRON MICROSCOPY[J]. Acta Phytopathologica Sinica, 1996, 26(3): 231-236 |
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| Authors: | Xu Junhuan Cheng Fangjing |
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| Affiliation: | 1. Depart. of Biological Science and Technology, Zhejiang Agricultural University, Hangzhou, China 310029;2. Biotech. Institute, Zhejiang Agricultural University, Hangzhou, China 310029 |
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| Abstract: | Attachment of virus particles to protein A-antiserum coated grids (portein Aimmunoelectron microscopy) provided a test about ten thousand times mo re sensitive than conventional electron microscopy or ten to one hundred times more sensitive than routine immunoelectron microscopy for detecting Ribgrass Mosaic Virus (RMV), Tobacco Mosaic Virus (TMV) in several vegetable crops. Antiserum dilution in 400, 000 times, infected sap dilution in 1, 000, 000 times and virus concentration of 2.5×10-7mg/ml produced satisfactory results. |
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| Keywords: | Vegetable virus diseases RMV TMV Immunoelectron microscopy |
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