| 犬新孢子虫NcSRS2基因片段的克隆及原核表达 |
| |
| 引用本文： | 丁德,贾立军,其木格,薛书江,张守发.犬新孢子虫NcSRS2基因片段的克隆及原核表达[J].河南农业科学,2008(5):111-113. |
| |
| 作者姓名： | 丁德 贾立军 其木格 薛书江 张守发 |
| |
| 作者单位： | 延边大学,农学院,吉林,龙井,133400 |
| |
| 摘 要： | 以含有犬新孢子虫NcSRS2基因的质粒pMD18-NcSRS2为模板,应用PCR方法扩增Nc-SRS2基因,将该基因片段克隆至原核表达载体pGEX-4T-2,构建了重组表达质粒pGEX-Nc-SRS2,转化大肠杆菌BL21中并诱导表达。结果显示,克隆的基因片段长1100bp,SDS-PAGE、Western blotting分析显示,重组质粒pGEX-NcSRS2在大肠杆菌中得到了高效表达。
|
| 关 键 词： | 犬新孢子虫 NcSRS2基因 克隆 原核表达 |
| 文章编号： | 1004-3268(2008)05-0111-03 |
| 修稿时间： | 2008年1月16日 |
Cloning and Prokaryotic Expression of NcSRS2 Gene of Neospora caninum |
| |
| DING De,JIA Li-jun,QI Mu-ge,XUE Shu-jiang,ZHANG Shou-fa.Cloning and Prokaryotic Expression of NcSRS2 Gene of Neospora caninum[J].Journal of Henan Agricultural Sciences,2008(5):111-113. |
| |
| Authors: | DING De JIA Li-jun QI Mu-ge XUE Shu-jiang ZHANG Shou-fa |
| |
| Abstract: | The NcSRS2 gene fragment of Neospora caninum was amplified from pMD18-NcSRS2 by PCR,and then inserte into the pGEX-4T-2 vector.The recombinant expression plasmid pGEX-NcSRS2 was successfully constructed.The gene was induced by IPTG to express in Escherichia coli BL21.Results showed that the NcSRS2 cDNA was 1100bp in length,SDS-PAGE、Western blotting analysis revealed that recombinant pGEX-NcSRS2 has been highly expressed in E.coli. |
| |
| Keywords: | Neospora caninum NcSRS2 gene Cloning Prokaryotic expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
