| 甘蓝型油菜隐性核不育基因的SRAP标记 |
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| 引用本文： | 陈锋,张洁夫,陈松,顾慧,戚存扣.甘蓝型油菜隐性核不育基因的SRAP标记[J].江苏农业学报,2007,23(4):283-288. |
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| 作者姓名： | 陈锋 张洁夫 陈松 顾慧 戚存扣 |
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| 作者单位： | 江苏省农业科学院经济作物研究所,江苏南京,210014;江苏省农业科学院经济作物研究所,江苏南京,210014;江苏省农业科学院经济作物研究所,江苏南京,210014;江苏省农业科学院经济作物研究所,江苏南京,210014;江苏省农业科学院经济作物研究所,江苏南京,210014 |
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| 基金项目： | 引进国际先进农业科技计划(948计划)
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江苏省高技术研究发展计划项目
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江苏省农业科学院资助项目 |
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| 摘 要： | 根据SRAP引物设计规律设计了一套引物,结合混合集群分析法(BSA),对油菜隐性细胞核雄性不育两用系430AB的不育与可育DNA池进行筛选,用在不育和可育DNA池间存在差异的引物再对430AB群体进行检测,获得了与隐性核不育基因ms连锁的3个SRAP标记,即m11e33270、m16e7280和m16e17370,它们位于核不育基因的同一侧,遗传图距分别为4.7 cM、20.1 cM和26.4 cM.
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| 关 键 词： | SRAP标记 甘蓝型油菜 核不育基因 |
| 文章编号： | 1000-4440（2007）04-0283-06 |
| 修稿时间： | 2006-10-23 |
SRAP Markers Linked to Recessive Genic Male Sterile Gene in Rapeseed (Brassica napus L.) |
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| CHEN Feng,ZHANG Jie-fu,CHEN Song,GU Hui,QI Cun-kou.SRAP Markers Linked to Recessive Genic Male Sterile Gene in Rapeseed (Brassica napus L.)[J].Jiangsu Journal of Agricultural Sciences,2007,23(4):283-288. |
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| Authors: | CHEN Feng ZHANG Jie-fu CHEN Song GU Hui QI Cun-kou |
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| Institution: | Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China |
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| Abstract: | A set of designed SRAP primers,combining with bulked segregation analysis(BSA) was used in a genic male sterile line 430AB of Brassica napus L.to identify the molecular markers linked to recessive genic male sterile gene.These SRAP primers which generated polymorphic bands between the DNA pools of male fertile and the male sterile plants were used to test the whole population of 135 individual plants of 430AB.Three SRAP markers,m11e33270,m16e7280 and m16e17370,were identified to be linked to the recessive genic male sterile gene.They were located at the same side of the male sterile gene with genetic distances of 4.7 cM,20.1 cM and 26.4 cM,respectively. |
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| Keywords: | SRAP marker Brassica napus L genic male sterile gene |
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