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Clonal propagation of Triticum durum Desf. from immature embryos and shoot base explants
Authors:O. A. Tanzarella  B. Greco
Affiliation:(1) Plant Breeding Institute, University of Bari, Italy;(2) Germplasm Institute, CNR, Bari, Italy
Abstract:Summary Immature embryos from five durum wheat cultivars were grown on Murashige and Skoog medium supplemented with two concentrations of kinetin or 6-benzylaminopurine (BAP). The embryos cultured on the medium containing 5 mg/l of BAP proliferated several axillary shoots. Shoot base segments subcultured on the same medium gave more shoot proliferation. The shoots developed into ear-bearing plants. This technique could be used for clonal propagation of wheat.
Keywords:Triticum durum  wheat  clonal propagation  cytokinins  shoot proliferation
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