远缘植物试管嫁接及ISSR分析

为了探明植物远缘嫁接的模式和分析嫁接后代的遗传特性，以大岩桐Sinningia speciosa，鸡冠花Celosia cristata，长寿花Kalanchoe blossfeldiana，黄色马蹄莲Zantedeschia elliottiana，百合Lilium longiflorum，苹果Malus domestica和阳桃Averrhoa carambola等7种植物为研究对象，采用试管嫁接的方法，进行了草本与木本植物、单子叶与双子叶植物、草本与草本植物的远缘嫁接，并对嫁接成活苗进行简单重复序列区间扩增多态性（inter-simple sequence repeat，ISSR）分析。结果显示，远缘植物之间的不同嫁接组合，嫁接的成活率有很大的差异，草本与草本植物之间、单子叶与双子叶植物之间进行嫁接较草本与木本植物之间的嫁接容易。ISSR分析表明，在大岩桐（接穗） ＋ 鸡冠花（砧木），鸡冠花 ＋ 大岩桐，长寿花 ＋ 大岩桐，长寿花 ＋ 鸡冠花4个组合中检测到部分嫁接成活苗有来自砧木与接穗的扩增位点，同时还具有不同于两者的特异扩增位点。图2表2参12

In vitro distant grafting with an ISSR analysis

To determine a model for in vitro distant grafting and to analyze genetic characteristics of descendants, seven plant species： Sinningia speciosa, Celosia cristata, Kalanchoe blossfeldiana, Zantedeschia elliottiana, Lilium longiflorum, Malus domestica, and Averrhoa carambola, were analyzed using inter simple sequence repeats （ISSR）. In vitro distant grafting was carried out between herbaceous plants and woody plants, monocotyledons and dicotyledons, and herbaceous plants and herbaceous plants. Results showed that grafting 1） herbaceous plants with herbaceous plants and 2） monocotyledons with dicotyledons had higher survival rates than 3） herbaceous plants grafted with woody plants. The ISSR markers indicated distant grafted plants from S. speciosa ＋ C. cristata, C. cristata ＋ S. speciosa, K. blossfeldiana ＋ S. speciosa, and K. blossfeldiana ＋ C. cristata that survived not only had scion and rootstock bands but also had their own unique bands.