Characterization of a phosphodiesterase component in a forest soil extract |
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Authors: | K. Hayano |
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Affiliation: | (1) Laboratory of Soil Biochemistry, National Institute of Agro-environmental Sciences, 305 Yatabe, Tsukuba, Ibaraki, Japan |
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Abstract: | Summary A bis-(p-nitrophenyl) phosphatase (BPN-Pase) was extracted from a forest soil and fractionated by DEAE-cellulose column chromatography into seven fractions (1, 2, 3, 4, 5, 6 and 7). The main fraction (fraction 5) was further fractionated into 3 subfraction (fractions 1, 2 and 3) by affinity chromatography for nuclease. The properties of the BPNPase in subfraction 3 were characterized and the results are reported in this article. Subfraction 3, which had a peak at about 278 run in the UV absorption spectrum, hydrolyzed 2,3-cyclic-nucleotides more readily than 3,5-cyclicnucleotides, adenylyl-(3 5)uridine, uridylyl-(3 5)adenosine, thymidine 3-p-nitrophenyl phosphate, thymidine 5-p-nitrophenyl phosphate, p-nitrophenyl phosphate and BPNP. Subfraction 3 hydrolyzed BPNP into 2 mol p-nitrophenyl and 1 mol inorganic phosphate during incubation. Apparent molecular weight of the BPNPase was estimated to be about 58 000 by gel filtration. The BPNPase activity had a pH optimum at 5.0 and was inhibited by Hg2+ and slightly inhibited by F– and PO43–.These observations suggest that the BPNPase is subfraction 3 has been constituted mainly with 2,3cyclic-nucleotide 2-phosphodiesterase [EC 3.1.4.16] or 2,3-cyclic-nucleotide 3-phosphodiesterase [EC 3.1.4.37]. |
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Keywords: | A bis-(p-nitrophenyl) phosphatase (BPNPase) Forest soil extract Fractionation on DEAE-cellulose column chromatography Oligonucleotides Cyclic nucleotides |
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