菌根真菌对铁皮石斛生长和矿质元素的影响

铁皮石斛组培苗接种6个真菌菌株,培养60 d后,接菌苗的平均鲜重增长率均高于对照,其中接种GS222、GDB162、GDB254、MLX102菌株的苗平均鲜重增长率均达到极显著差异(α=0.01),接种CLN103、CLX104的苗达到显著差异(α=0.05);接菌苗的各矿质元素(除Zn外)含量也均高于对照。接菌苗的营养根重分离获得原接种菌株。显微观察到在皮层和中柱组织形成菌丝结等典型的共生结构,表明接种菌株已与铁皮石斛根共生形成了菌根,对铁皮石斛的生长和矿质元素的吸收有较明显促进作用。由此可认为GS222、GDB162、GDB254、MLX102、CLN103、CLX104是铁皮石斛优良的菌根菌株。

Effects of mycorrhizal fungi on the growth and mineral nutrition absorption of Dendrobium candidum

Six strains were inoculated into the tissue culture seedlings of Dendrobium candidum.After 60 days,the average fresh weights of seedlings had been higher than those of the control.In detail: The average fresh weight of seedlings,inoculated with GS222,GDB162,GDB254,MLX102,had been extremely significantly increased (α=0.01),and inoculated with CLN103,CLX104,had been significantly increased(α=0.05).The mineral elements contents of the seedlings had also been higher than those of the control ((except Zn).)And the fungal strains had already been reisolated from the mycorrhiza.It was found that there were pelotons in cortical and vascular tissue by the observation of the microstructure of mycorrhiza.Therefore it was confirmed that the(relationship) of symbiosis had been formed,the growth and mineral nutrition absorption of D.candidum had been promoted by the mycorrhizal fungi.So it was proved that GS222,GDB162,GDB254,MLX102,CLN103,CLX104 were good mycorrhizal strains of D.candidum.