| 60Coγ射线对辅酶Q10产生菌三孢布拉氏霉的诱变选育 |
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| 引用本文： | 吴品芳,陆茂林. 60Coγ射线对辅酶Q10产生菌三孢布拉氏霉的诱变选育[J]. 西北农林科技大学学报(自然科学版), 2006, 34(4): 73-75 |
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| 作者姓名： | 吴品芳 陆茂林 |
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| 作者单位： | 1. 江南大学,生物工程学院,江苏,无锡,214036
2. 江苏省微生物研究所,江苏,无锡,214063 |
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| 摘 要： | 采用不同剂量的60Coγ射线对辅酶Q10产生菌——三孢布拉氏霉JSF 4菌株的孢子进行处理。结果表明,诱变剂量1.5 kG y对孢子的致死率为85%,具有较好的诱变效果。经复筛,分离得到了1株F 4-3菌株,其发酵平均单位达24.766 m g/L,较出发菌株提高了76%,且该菌株遗传性状稳定。
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| 关 键 词： | 60Coγ射线 辅酶Q10 三孢布拉氏霉 |
| 文章编号： | 1671-9387(2006)04-0073-03 |
| 收稿时间： | 2005-10-24 |
| 修稿时间： | 2005-10-24 |
Studies on the screening of mutant of Blakeslea trispora on CoQ10 induced by 60Coγ radiation |
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| WU Pin-fang,LU Mao-lin. Studies on the screening of mutant of Blakeslea trispora on CoQ10 induced by 60Coγ radiation[J]. Journal of Northwest A&F University（Natural Science Edition）, 2006, 34(4): 73-75 |
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| Authors: | WU Pin-fang LU Mao-lin |
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| Affiliation: | 1. School of Biotechnology,Southern Yangtze University,Wuxi, Jiangsu 214036,China; 2 .Jiangsu Institute of Microbialogy Co. Ltd. Wu xi, Jiangsu 214063, China |
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| Abstract: | The spore suspension of Blakeslea trispora was treated with different dosages of ^60Co irradia tion. The study showed that when 1.5 kGy mutagenic dosage was provided, 85 % of the spores died,resulting in a good mutagenic effect. By re-screening,a mutant F4-3 was obtained. Compared with the parent strain,its production of CoQ10 reached 24. 766 mg/L,and the increase rate was 76% ,and its genetic character was stable. |
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