植物有机营养无菌培养试验方法的研究与应用

本文对植物有机营养研究中无菌培养实验室的结构，功能作了描述，对有机营养无菌水培液的配制及无蓝图苗的筛选和培育方法作了检验。用此无菌试验法进行的水稻有机氮营养试验证明，水稻能直接吸收根际营养液中的氨基酸，在频繁更换营养液的培养条件下，等氮量有机、无机 氮对对水稻的营养效果依次为甘氨酸态（无菌条件）〉谷氨酸态氮（无菌条件）〉铵态氮（无菌条件）〉铵态氮（常规条件），反映出有机、无机氮种类及无菌、常规试验

STUDY OF STERILE CULTURE METHOD FOR RESEARCHING PLANT ORGANIC NUTRITION AND ITS APPLICATION

This paper describes the structure and function of plant sterile culture laboratory in details. Some key steps in plant sterile culture such as sterilization of nutrient solution and culture of aseptic seedlings were tested successfully. Results from sterile experiment for rice organic N nutrition showed that amino acid could be directly taken up by plants. The effectiveness of N sources at equal N concentration (N 10mg/L) for rice followed the order: glycine-N (sterile culture)>glutamic acid-N (sterile culture)>ammonium sulphate-N (sterile culture)>ammonium sulphate-N (conventional culture) under frequent renewal of nutrient solution in the experiment. This indicated clearly the differences among the effciency of various organic and inorganic N compounds, and sterile and conventional growth conditions on rice N nutrition.