| 佛甲草中SOD提取条件的优化 |
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| 引用本文： | 陈莉,申小蓉,赵红洋,刘括.佛甲草中SOD提取条件的优化[J].草原与草坪,2009(1). |
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| 作者姓名： | 陈莉 申小蓉 赵红洋 刘括 |
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| 作者单位： | 1. 西北名族大学生命科学与工程学院,甘肃,兰州,730020
2. 梦绿公司,甘肃,兰州,730020 |
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| 基金项目： | 甘肃省自然科学基金，全球环境基金 |
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| 摘 要： | 对景天科植物佛甲草超氧化物歧化酶(SOD)的抽提缓冲液pH值,抽提时间,热处理的温度和时间进行正交实验,并对丙酮沉淀法和硫酸铵盐析法初步纯化SOD粗酶液进行了优化和比较。结果表明:抽提缓冲液的pH值为7.8,抽提时间为2 h,热处理温度为50℃,热处理时间为15 min时,得到佛甲草SOD的相对最大提取酶活力;饱和度为90%的硫酸铵盐析是初步纯化佛甲草SOD的最优条件,其纯化倍数为4.10,回收率为0.71,为进一步的层析以制备更纯佛甲草SOD提供了理论的条件。
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| 关 键 词： | 佛甲草 SOD 提取条件 纯化 |
Optimization of treatment conditions for extracting SOD in Sedum lineare |
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| CHEN Li,SHEN Xiao-rong,ZHAO Hong-yang,LIU Kuo.Optimization of treatment conditions for extracting SOD in Sedum lineare[J].Grassland and Turf,2009(1). |
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| Authors: | CHEN Li SHEN Xiao-rong ZHAO Hong-yang LIU Kuo |
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| Institution: | 1.College of Life Science and Engineering;Northwest University for Nationalities;Lanzhou 730020;China;2.Gansu Menglu Environment Engineering Ltd.;China |
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| Abstract: | The factors affecting SOD extraction,including pH value of buffer,extraction time,temperature and time of heating treatment,were studied by using Sedum lineare with orthogonal test.The results showed that the activity of SOD was the highest under the following treatment conditions: buffer pH 7.8,extracting for 120 min,heat treatment for 15 min at 50 ℃.Meanwhile,the best condition for SOD purification was 90% of ammonium sulfate,and its purification multiplier and recovery rate were 4.10 and 0.71 respectively. |
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| Keywords: | SOD |
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