微孔草扩繁和栽培技术研究

研究并确定微孔草茎尖是组织培养的最佳部位,细胞分裂素(6-BA)最适浓度是3mg/L。经初代培养、继代扩繁、温室育苗等阶段形成的块根,供大田栽培,生产种子扩大繁殖。育苗区的种植密度是200株/m²,收种区25株/m²。同时制定"三区"和"四区"轮作方案及播前土壤处理方法,有效地控制杂草危害,提高了种子生产量。

Study on the Technique of Plant Microula Bench

The study ascertains that the tissue of the tip of a Microula bench plant is best for its quick regeneration and that the concentration of the MS basic medium with different plant hormones should be 6-Bais 3 mg/L. After the first generation culture and the successive multiplication, the Microula bench grass was planted in the soil to grow root tubers.The study shows that based on the nutriment for growth and reproduction, the density of Microula bench plants in the nursery plot should be 200 plants/m~2, and in the seed-collection plot 25 plants/m~2. Using a thousand grams of Microula bench seed to calculate the plant density in a piece of land, it is 200 plants/m~2 for the nursery plot, and 25 plants/m~2 for the seed collecting plot. Moreover, the actual seed grains for the seed collecting plot and the additional seeds needed based on the different ecological conditions have also been calculated. To prevent rank grasses growing in the plots, direct sowing and crop rotation of seedling nursing, seed collection and root tuber planting were adopted. Satisfactory results were also achieved by using four plots for crop rotation.