| 天冬氨酸激酶工程菌发酵条件的响应面优化研究 |
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| 引用本文： | 汪一名,闵伟红,刘嘉. 天冬氨酸激酶工程菌发酵条件的响应面优化研究[J]. 农产品加工．学刊, 2011, 0(7): 54-59. DOI: 10.3969/j.issn.1671-9646(X).2011.07.015 |
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| 作者姓名： | 汪一名 闵伟红 刘嘉 |
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| 作者单位： | 吉林农业大学食品科学与工程学院,吉林长春,130018 |
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| 摘 要： | 对天冬氨酸激酶工程菌的生长情况及发酵的最佳条件进行了系统研究,探讨了发酵温度、发酵液pH值、摇床转速、接种量等因素对发酵的影响。在单因素试验的基础上,利用中心组合试验和响应面分析法确定了天冬氨酸激酶工程菌发酵的最佳条件为:发酵温度37℃,发酵液pH值7.5,摇床转速180 r/min,接种量2%。天冬氨酸激酶活性达到最大值201 U/mL。
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| 关 键 词： | 天冬氨酸激酶 发酵 响应面优化 |
Response Surface Optimization of Aspartokinase Recombinant Fermentation |
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| Wang Yiming,Min Weihong,Liu Jia. Response Surface Optimization of Aspartokinase Recombinant Fermentation[J]. Nongchanpin Jlagong.Xuekan, 2011, 0(7): 54-59. DOI: 10.3969/j.issn.1671-9646(X).2011.07.015 |
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| Authors: | Wang Yiming Min Weihong Liu Jia |
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| Affiliation: | Wang Yiming,Min Weihong,Liu Jia(Food Engineer College,Jilin Agricultural University,Changchun,Jilin 130018,China) |
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| Abstract: | In this study,the growth condition and optimal fermentation conditions are determined to explore the temperature,pH,rotate speed,inoculum and other single factor on the fermentation of recombinant E.coli.Based on single-factor experiments,central composite experiments and response surface analysis are applied to determine the optimal fermentation conditions: the temperature is 37 ℃,pH value is 7.5,rotate speed is 180 r/min,2% of inoculums.Aspartokinase activity reach to maximum 201 U/mL. |
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| Keywords: | Aspartokinase fermentation response surface optimization. |
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