| L-苏氨酸生产菌的选育 |
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| 引用本文： | 张春,程丽,闵伟红.L-苏氨酸生产菌的选育[J].农产品加工．学刊,2011(7):77-81. |
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| 作者姓名： | 张春 程丽 闵伟红 |
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| 作者单位： | 吉林农业大学食品科学与工程学院,吉林长春,130118 |
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| 摘 要： | 以北京棒杆菌AS 1.299(Corynebacterium pekinense)为出发菌株,经过紫外—亚硝基胍(NTG)诱变,得到L-苏氨酸高产突变株N50-10,其质量浓度为1.150 g/L,比原菌株质量浓度提高了1.106 g/L。在菌株N50-10的基础上,又筛选出抗乙硫氨酸和抗磺胺胍突变株SG06-10(Ethr+SGr),其L-苏氨酸质量浓度达到了2.463 g/L,比原菌提高了2.419 g/L。经5次传代,产量稳定。
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| 关 键 词： | L-苏氨酸 诱变 抗性突变株 |
Breeding of Producing L-threonine Strain |
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| Zhang Chun,Cheng Li,Min Weihong.Breeding of Producing L-threonine Strain[J].Nongchanpin Jlagong.Xuekan,2011(7):77-81. |
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| Authors: | Zhang Chun Cheng Li Min Weihong |
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| Institution: | Zhang Chun,Cheng Li,Min Weihong(Food Engineer College,Jilin Agricultural University,Changchun,Jilin 130118,China) |
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| Abstract: | Taking Corynebacterium pekinense AS1.299 as starting strain,a high producing strain N50-10 is obtained by mutagenizing UV and NTG,its volume of production is 1.150 g/L.And ethionine resistance and sulfaguanidine resistance Strain SG06-10(Ethr+SGr) are screened out on the basis of strain N50-10,which can bring L-threonine up to 2.410 g/L,comparing with Corynebacterium pekinense AS1.299 has 2.366 g/L with increasing,it shows that go down to 5 posterity has good genetic stability. |
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| Keywords: | L-threonine mutagenesis resistant mutants |
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