大豆异黄酮抵抗体外培养猪脂肪细胞氧化损伤的作用

为了探明大豆异黄酮对猪脂肪细胞氧化损伤的保护效应,试验分别用含0、10、20、40μmol/L和80μmol/L异黄酮S(一种合成的大豆异黄酮)或三羟基异黄酮(GEN)的完全培养液培养猪脂肪细胞48h,用终浓度为100μmol/L的FeSO4和H2O2溶液进行氧化处理1 h。结果表明:与正常对照组相比,氧化对照组细胞内活性氧(ROS)水平和脂质过氧化产物丙二醛(MDA)含量分别升高了1.61倍和2.74倍(P<0.01);与氧化对照组相比,添加10、20、40μmol/L和80μmol/L异黄酮S使细胞内ROS水平分别下降了24.62%(P<0.05)、20.03%(P<0.05)、37.88%(P<0.01)和41.20%(P<0.01);添加10、20、40μmol/L和80μmol/L GEN均显著降低了细胞内ROS水平(P<0.01)和MDA含量(P<0.05)。试验结果提示在本试验条件下,异黄酮S和GEN能通过抑制猪脂肪细胞的脂质过氧化、降低ROS产生,抵抗羟自由基对猪脂肪细胞的氧化损伤。

The Propective Effects of Soybean Isoflavones against Oxidative Damage to Porcine Adipocytes in vitro

Porcine adipocytes were exposed to a synthetic isoflavone(ISO-S)or genistein(GEN) at different concentrations(0,10,20,40 and 80 μmol/L) for 48 h,respectively.Then the medium was changed to one containing 100 μmol/L FeSO4 and H2O2.Cells were incubated for another 1 h before sampling to evaluate the protective effects of soybean isoflavones against oxidative damage to adipocytes.Two-factorial ANOVA revealed that reactive oxygen species(ROS) levels and malondialdehyde(MDA) contents in porcine adipocytes treated by Fe2+/H2O2 system were influenced by the concentrations of soybean isoflavone(P < 0.05).Compared to the control cells,the cellular levels of ROS and MDA in oxidized control group were increased by 1.61 times(P < 0.01) and 2.74 times(P < 0.01),respectively.When adipocytes were preincubated with 10,20,40 and 80 μmol/L ISO-S,the cellular level of ROS was decreased by 24.62%(P < 0.05),20.03%(P < 0.05),37.88%(P < 0.01) and 41.20%(P < 0.01) respectively,with respect to the oxidized control.Preincubation with GEN(10,20,40 and 80 μmol/L) significantly decreased the levels of ROS(P < 0.01) and MDA(P < 0.05) in adipocytes,compared with the oxidized control.These results indicated that preincubation with ISO-S or GEN could alleviate oxidative injury of porcine adipocytes induced by hydroxyl radical through decreasing lipid peroxidation and ROS production.