稗内脐蠕孢菌（Drechslera monoceras）原生质体制备和再生

摘要：研究了菌龄、酶浓度、渗透压稳定剂、缓冲液pH值以及酶解温度和时间等因子对稗内脐蠕孢菌（Drechslera monoceras）原生质体形成的影响。将培养42 h的菌丝，以0.7 mol/L KCl作为渗透压稳定剂，在30 ℃, pH 4.4～5.8条件下，经2%溶壁酶和2%纤维素酶混合酶液酶解4 h，静止条件下原生质体最大释放量达1×106/mL,再生率为0.15%。

Preparation and Regenaration of Drechslera monoceras Protoplast

Abstract: Factors, which affect the protoplast formation of barnyardgrass (Echinochloa spp.) pathogen Drechslera monoceras, such as mycelium age, lytic enzyme concentration, osmotic pressure of stabilizer, pH value of buffer solution, digesting temperature, period and so on, were studied. Maximum yield (1×106/mL) of protoplast could be obtained with 42 h old mycelia by using 2% lywallzyme in combination with 2% cellulase which containing 0.7 mol/L KCl as osmotic stabilizer and digesting for 4 h at 30 ℃ in pH 4.4~5.8 condition. The regeneration rate of the protoplast cultured on the solid media containing osmotic stabilizer was 0.15%.