| HPLC色谱法测定贵州黔北凹叶厚朴中厚朴酚的含量 |
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| 引用本文： | 冯华,王样培,邹孔强,王小东. HPLC色谱法测定贵州黔北凹叶厚朴中厚朴酚的含量[J]. 安徽农业科学, 2010, 38(15): 7841-7841,7865. DOI: 10.3969/j.issn.0517-6611.2010.15.051 |
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| 作者姓名： | 冯华 王样培 邹孔强 王小东 |
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| 作者单位： | 遵义市药品检验所,贵州遵义,563002;贵阳中医学院,贵州贵阳,553002 |
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| 基金项目： | 遵义市中药现代化科技产业研究开发专项项目 |
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| 摘 要： | [目的]建立用高效液相色谱法测定贵州黔北凹叶厚朴中厚朴酚的含量。[方法]色谱柱为Diamonsil C18,以甲醇-水（78∶22）为流动相;流速为1.0ml/min;柱温25℃;检测波长为294nm。[结果]厚朴酚线性范围为0.6272～1.1648ug,r=0.9994;平均回收率为98.56%,RSD=0.33%。[结论]该法简便、灵敏、准确。
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| 关 键 词： | 高效液相色谱法 黔北凹叶厚朴 厚朴酚 含量测定 |
Content Determination of Magnolol in Qianbei Magnolia officinalis by HPLC |
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| FENG Hua et al. Content Determination of Magnolol in Qianbei Magnolia officinalis by HPLC[J]. Journal of Anhui Agricultural Sciences, 2010, 38(15): 7841-7841,7865. DOI: 10.3969/j.issn.0517-6611.2010.15.051 |
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| Authors: | FENG Hua et al |
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| Affiliation: | FENG Hua et al(Zunyi Institute for Drug Control,Zunyi,Guizhou 563002) |
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| Abstract: | [Objective] The aim was to determine the content of magnolol in Qianbei Magnolia officinalis by HPLC method.[Method] A Diamonsil C18 column was used with mobile phase of methanol-water(78∶22),the detection wavelength was 294 nm,the flow rate was 1.0 ml/min,the column temperature was 25 ℃.[Result] The magnolol good liner range was within 0.627 2-1.164 8 ug,r=0.999 4.The average recovery was 98.56% and RSD was 0.33%.[Conclusion] The method is simple,sensitive and accurate. |
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| Keywords: | HPLC Qianbei Magnolia officinalis Magnolol Content determination |
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