高压脉冲电场辅助提取河蚌多糖工艺优化

利用高压脉冲电场从河蚌中辅助提取河蚌多糖来提高多糖得率。进行了单因素试验和Box-Behnken设计试验，结果表明，电场强度和脉冲数对河蚌多糖得率影响显著，通过建立多糖得率与各影响因子之间关系的回归数学模型，确定高压脉冲电场辅助提取河蚌多糖的最佳工艺参数为电场强度25kV/cm、脉冲数8、液料比40mL/g，此时处理2g样品所得多糖占样品湿质量的4.99%。与超声波提取河蚌多糖对比试验表明，高压脉冲电场辅助提取河蚌多糖的得率较超声波提取提高了11.9%，且其处理时间较短。

Process Optimization for Extraction of Mussel Polysaccharides Assisted by High Intensity Pulsed Electric Fields

The high intensity pulsed electric fields (PEF) technique was applied for assisted extraction of mussel polysaccharides to improve the efficiency of mussel polysaccharides extraction. The single factor test and Box Behnken design results showed that electrical field strength and pulse number were the significant affecting factors on the extraction efficiency of mussel polysaccharides. With mathematical regression model analysis of extraction efficiency and the impact factor, the optimal combination of parameters was found that electrical field strength was 25kV/cm, pulse number was 8, water powder ratio was 40mL/g. Under the optimized condition, the extraction rate of 2g mussel was up to 4.99% (polysaccharides wet weight sample ratio). Compared with ultrasonic technique, high intensity pulse electric fields is less time consuming with higher extraction efficiency. The study can provide a reference for further studies of extraction of mussel polysaccharides.