小菜蛾气味结合蛋白PxylOBP13基因克隆与序列分析

小菜蛾是为害十字花科蔬菜的重要害虫,干扰其嗅觉识别功能是实现小菜蛾有效治理的重要途径。本研究克隆并鉴定了1个小菜蛾气味结合蛋白基因,命名为PxylOBP13(GenBank登录号为KT156679);该基因开放阅读框全长387bp,编码128个氨基酸;预测蛋白分子量为14.33KDa,等电点为7.55,N端无信号肽序列,有3个疏水性区域,具有气味结合蛋白家族的保守结构域和6个保守的半胱氨酸残基,与6种鳞翅目昆虫的气味结合蛋白氨基酸序列一致性达50%以上。研究结果可为进一步研究小菜蛾气味结合蛋白功能,阐明小菜蛾嗅觉机制,制定科学有效的治理措施提供依据。

Cloning and Sequence Analysis of an Odorant Binding Protein Gene PxylOBP 13 in the Diamondback Moth,Plutella xylostella (Lepidoptera:Plutellidae)

The diamondback moth (DBM),P lutella xylostella (L.)is a key insect pest on cruciferous plants all over the world. Olfactory perception is critical for survival and reproduction in insects,and correspondingly provides a new target to control DBM. Hereby,an odorant binding protein (OBP)gene from P.xylostella,PxylOBP 13 (GenBank accession number:KT1 56679 )was cloned;the bio-information characteristics were analyzed;and a phylogenetic tree was constructed. The results showed that the full-length of open reading frame of PxylOBP 13 is 387 bp and encodes 128 amino acids;PxylOBP 13 contains 6 conserved cysteine residues and the conserved domain of odorant binding protein family,and 3 hydrophobic regions which are potential binding sites for hydrophobic odorants. The protein molecular mass was predicted as 14.33 kDa with an isoelectric point of 7.55,but the N-terminal without signal peptide;the similarity of sequence between PxylOBP 13 and six lepidopteran insects can reach above 50%. The results can provide theoretical support for further research on the function of OBPs in DBM and development of effective management of DBM based on olfactory mechanism.