BDE-47胁迫对双齿围沙蚕抗氧化防御系统的影响

将双齿围沙蚕Perinereis aibuhitensis暴露在BDE-47下进行急性毒性试验,测定96h-LC_(50)。在此基础上,研究了暴露在0、2、4、8mg·L~(-1)的BDE-47污染物下,双齿围沙蚕的谷胱甘肽(GSH)含量、谷胱甘肽过氧化物酶(GPx)活力、超氧化物歧化酶(SOD)活力、过氧化氢酶(CAT)活力以及丙二醛(MDA)含量、谷胱甘肽硫转移酶(GST)活力的变化情况。结果表明BDE-47对双齿围沙蚕的96h-LC_(50)为31.24mg·L~(-1)。在暴露期间,与对照组比较,各处理组的GSH含量、GST活力、CAT活力总体上无显著变化,仅8mg·L~(-1)组的GST活力在14d极显著性升高(P0.01)。暴露1d时,4mg·L~(-1)处理组GPx活力显著升高(P0.05),在第7~14d时,2mg·L~(-1)处理组的GPx活性呈上升趋势;在暴露1d时,4 mg·L~(-1)、8 mg·L~(-1)两组的SOD活力显著下降(P0.05),整体上看,在1~7d时,SOD活力被诱导升高。14d时,与对照组的比较,4、8mg·L~(-1)处理组的SOD活力分别被显著(P0.05)和极显著(P0.01)抑制。在暴露1d时,MDA含量被诱导升高(P0.05),且4mg·L~(-1)极显著升高(P0.01)。在暴露4~14dMDA含量总体上无明显变化,仅第7d的8mg·L~(-1)处理组显著升高(P0.05)。结论:在双齿围沙蚕抗氧化防御系统中,MDA含量和SOD活力均在胁迫初期有显著变化,因此MDA含量和SOD活力可以作为潜在的生物标志物。

Effect of the Antioxidant Dfense Sstem of Perinereis aibuhitensis Exposed to Tetrabromodiphenyl Ether

Perinereis aibuhitensis is used as biological subject in acute toxicity test,for determining the 96 h-LC50 of tetrabromodiphenyl ether(BDE-47).On this basis,the activities of SOD,CAT ,GST,GPx and the contents of GSH,MDA of the antioxidant defense system of Perinereis aibuhitensis exposed to 0,2,4,8 mg · L-1 of BDE-47 pollutants were determined.The results were as follows:the 96h-LC50 of BDE-47 to Perinereis aibuhitensis was 31.24 mg · L-1 . Compared with the control group,the content of GSH and activities of GST and CAT had basically no significant change, only the GST activity of 8 mg·L-1 group was significantly higher (P <0.01)on 14 th day.The GPx activity of 4 mg· L-1 group was significantly higher (P <0.05)on 1 th day,and 2 mg·L-1 group showed the increasing trend during the 7 th to 14 th day.On 1 th day,the SOD activity of the 4 mg·L-1 and 8 mg·L-1 groups significantly decreased (P <0.05). Overall,during the period of 1-7 d,SOD activity of the 2 mg·L-1 ,4 mg·L-1 groups were induced to rise. On 14 th day,SOD activity in 4 mg·L-1 ,8 mg·L-1 groups were significant (P <0.05)and significantly (P <0.01)inhibited respectively. The MDA content of all the treatment groups were induced to increase (P <0.05),and 4 mg·L-1 group significantly increased (P <0.01)on 1 th day. There was no significant change in the content of MDA of the treatment groups during the exposure period of 4-14 th day,only 8 mg·L-1 group was significantly higher (P <0.05)on 7 th day.In conclusion:the content of MDA and activity of SOD of the antioxidant defense system of Perinereis aibuhitensis had significant changes at the early exposure stage (P <0.05),which could be used as potential biomarkers.