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| 马铃薯转基因受体系统的建立 | |
| 引用本文： | 徐美隆,秦彬彬,江佰阳,吴建华. 马铃薯转基因受体系统的建立[J]. 黑龙江农业科学, 2009, 0(6): 1-3,8 |
| 作者姓名： | 徐美隆 秦彬彬 江佰阳 吴建华 |
| 作者单位： | 1. 种苗生物工程国家重点实验室,银川,宁夏,750004 2. 国家经济林木种苗快繁工程技术研究中心,银川,宁夏,750004 3. 种苗生物工程国家重点实验室,银川,宁夏,750004;宁夏大学,生命科学学院,银川,宁夏,750021 |
| 摘 要： | 以马铃薯（Solanumtuberosum L.）“Wen-40”和“紫花白”2个品种的茎段和叶片为外植体,通过离体培养分化成苗以及对卡那霉素（Km）和头孢霉素（Cef）的敏感性研究,建立了农杆茵介导的稳定、高效的基因转化受体系统。结果表明：“Wen-40”的茎段适合转基因高效再生体系的建立,而25 mg.L^-1的Km适合转基因植株的抗性筛选,低于200 mg.L^-1的Cef浓度对马铃薯的诱导分化影响较小。 |
| 关 键 词： | 马铃薯 转基因 受体系统 |
Establishment of a Transgenic Acceptor System for Solanum Tuberosum L. |
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| XU Mei-long,QIN Bin-bin,Jiang Bai-yang,WU Jian-hua. Establishment of a Transgenic Acceptor System for Solanum Tuberosum L.[J]. Heilongjiang Agricultural Science, 2009, 0(6): 1-3,8 | |
| Authors: | XU Mei-long QIN Bin-bin Jiang Bai-yang WU Jian-hua |
| Affiliation: | XU Mei-long ,QIN Bin-bin ,Jiang Bai-yang ,WU Jian-hua (1. State Key Laboratory of Seedling Bioengineering, Yinchuan, Ningxia 750004;2. National Center of Economic Forest Seedling Speediness Propagation Engineering and Technology, Yinchuan, Ningxia 750004; 3. Life Science College of Ningxia University,Yinchuan,Ningxia 750021) |
| Abstract: | Stem segments and blades were used as explants of tissue culture of Solanum tuberosum L.varieties"Wen-40"and"Zihuabai",plantlet regeneration and effects of antibiotics,including kanamycin（Km）,and cefotaxime（Cef）,on the induction of callus were studied.The results indicated that stem segments was more suitable for establishment of regeneration system than blades.The optimized concentration of kanamycin for selecting the stem segments was 25 mg · L~(-1) and cefotaxime was less than 200 mg · L~(-1). |
| Keywords: | Solanum tuberosum L. transgene receptor system |
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