转基因香蕉植株对根际土壤微生物的影响

通过对遗传转化技术获得的转基因香蕉植株和对照植株根际土壤微生物的培养实验,结果表明,转基因植株和对照植株对根际土壤中细菌、真菌、放线菌种群均没有影响.土壤微生物在BIOLOG GN微平板上的ELISA反应结果表明,转基因香蕉植株根际土壤微生物的每孔颜色平均变化率(Average Well Color Devel-opment,AWCD)的变化曲线和对照AWCD的变化曲线接近吻合.进一步对72 h的土壤微生物群落功能多样性的5种指数作方差分析,结果表明,5种指数和对照没有显著差异.实验结果表明转基因香蕉植株和对照植株对根际土壤微生物群落的结构和功能多样性没有产生影响.

Effect of Transgenic Banana Plants on the Microorganism in the Soil

An experiment was carried out to compare the diversity and structure of microorganism in the soil around the roots of the transgenic banana plants and the controls. The result showed no difference in the communities of bacteria, fungi and actinomycetes between the transgenic banana plants and the controls. Enzyme-linked immunosorbent assay(ELISA) of Average Well Color Development(AWCD) indicated that the AWCD curve of the transgenic banana nearly fitted that of the controls. Analysis of 5 indexes of the diversity of the soil at 72 h through Duncan's multiple-range test indicated no difference between the transgenic and control plants, suggesting that the transgenic banana plants did not affect the diversity and structure of the micro-organism in the soil around the roots.